BBa_I716332 1 BBa_I716332 ThpB 2007-07-10T11:00:00Z 2015-08-31T04:07:52Z ThpB From IGEM07 PCR ThpBF/ ThpBIR on ThpB (610 bp, gp=A) PCR ThpBR/ ThpBIF on ThpB (712 bp, gp=B) -------------------------------------------------------------------------------- PCR ThpBAF/ThpBAR on A+B (1285bp BglII/XhoI) Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L) Product pBca9145-BBa I716332 -------------------------------------------------------------------------------- ThpBF Forward BglII site anneals to ThpB ctagaAGATCTgtgaccatcaccccgcccgc ThpBIF Removing XhoI site in ThpB ggttcgagcggctcctTgaggaccagggctccggac ThpBIR Removing XhoI site in ThpD gtccggagccctggtcctcAaggagccgctcgaacc ThpBR Reverse XhoI site anneals to ThpB cgtcactcgagtcaggccgtttcgcggacgc This gene is very similar to OtsA and OtsB, and this will help prevent dehydration. false false _110_ 0 2095 9 It's complicated false ((BerkiGEM2007-Biobrick2.0Intro)) false nhu nguyen BBa_I716332_sequence 1 accttagctttcgctaggatgatttctggaattcatgagatctgtgaccatcaccccgcccgccctgagcgtcttcgagaccctggagtccgaggtacggagctactgccgcggctggcccgccgtgttcgaccgcgcgcagggcgcccggctgaccgacgaggacggccactcctacctggacttcttcgccggggccggctcgctcaactacggccacaacaacccggtgctgaaacgagcgctgatcgactacatcgagcgcgacggcatcacccacggcctggacatggcgaccaccgccaagcgggcgttcctggagacgttccagaacgtgatcctgcggccgcgcgatctgccgtacaaggtgatgttccccggcccgaccggcaccaacgccgtcgagtcggcgctgaagctggcccgcaaggtcaagggccgcgagtcggtcgtctcgttcaccaacgccttccacggcatgtcgctgggctcgctcgcggtgaccgggaacgcgttcaagcgggccggggccggtatcccgctggtccacggcaccccgatgccgttcgacaactacttcgacggcacggtccccgacttcctctggttcgagcggctccttgaggaccagggctccggactcaacaagcccgcagccgtgatcgtggagaccgtccagggcgagggcggcatcaacgtcgcccgcgccgagtggctgcgcgcgctccaggagctctgccaccgccaggacatgctgctgatcgtcgacgacatccagatgggctgcggccgcaccggcggcttcttctccttcgaggaggccggcatcgtcccggacatcgtcacgctctccaagtccatcagcggctacgggcctgcccatgtccctctgcctcttcaagcccgagctgggacatctggggagcccgggcgagcacaacgggcacctttccgcggcaacaaccggtcttcgtcacggcgcgtcgtgctcgacgcttactggtcgacgcagatggagaagcagaccctcgcccgcggcgacaggtcgaacgatcctggctcgcatctgctcgagacgaactgccagttcccgcggctggtggggcaatgatcagtacaggatcgctcctagacgtatgggactcggtc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z