BBa_I716354 1 BBa_I716354 Biobricked Unmodified Hemoglobin Cassette in petDUET format 2007-10-23T11:00:00Z 2015-08-31T04:07:52Z Pre-biobricking modifications to I716091. Quickchange AD0013F/R to get rid of EcoR1 site. Quickchange AD0014/F/R to get rid of BglII site. Quickchange AD0015F/R to get rid of XhoI site. PCR AD0016F/R on modified I716091. Digest Bgl2/Xho1 and insert into 9145 plasmid. Product is I716354 Beta and alpha HbA subunits inserted into the commercial petDUET vector. The vector has each coding sequencing under separate T7 promoters. false false _110_ 0 1286 95 It's complicated false {{BerkiGEM2007-Biobrick2.0Intro}} false Austin Day BBa_I716354_sequence 1 gaattcatgagatctgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatggtacacttaaccccggaagaaaaatctgcggtgactgctttatggggtaaagttaacgtggatgaagttggtggtgaggcgttgggccgtctgctggttgtgtatccttggacacagcgctttttcgagagctttggtgacctgtctactcccgatgcggtgatgggaaacccgaaagtaaaagcgcatggcaaaaaggtgttgggcgcgttctcggatggtctggcgcatcttgataatcttaaaggtacattcgcgactctgagtgaactccattgtgacaagctccatgttgatcctgagaattttcgtctgctgggtaacgtgctggtgtgtgtccttgcgcatcatttcggtaaggaatttaccccgccggtacaggcggcctatcagaaagtggttgcgggtgtagccaatgcgctggcccataaatatcattaagatttcgagctcggcgcgcctgcaggtcgacaagcttgcggccgcataatgcttaagtcgaacagaaagtaatcgtattgtacacggccgcataatcgaaattaatacgactcactataggggaattgtgagcggataacaattccccatcttagtatattagttaagtataagaaggagatatacatatggttctgtccccagctgataaaacaaacgtaaaagcggcttggggaaaagtcggcgcacacgcgggtgagtatggtgctgaagctttagaacgcatgtttttaagctttcctaccaccaaaacctacttcccgcacttcgatttatcacatgggtcagcacaggtaaagggccacggcaagaaagtagccgacgcgctcaccaacgccgttgcccacgtagacgatatgccgaatgccctgtctgcgctgagcgacctgcatgcgcataaactccgtgtggacccggttaactttaaactgctgtcccactgtctgctggtaaccctcgcggctcatttgccggcagagtttacgccagcggtgcatgcatccctcgataaattcttggcgtcagtgagcacggtgcttaccagcaagtatcgttaaagttctcaattggatatcggccggccacgcgatcgctgacgtcggtacccttgagtctggtaaagaaaccgctgctgcgaaatttgaacgccagcacatggactcgtctactagcgcagcttaattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgggatccttactcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z