BBa_I716354
1
BBa_I716354
Biobricked Unmodified Hemoglobin Cassette in petDUET format
2007-10-23T11:00:00Z
2015-08-31T04:07:52Z
Pre-biobricking modifications to I716091.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.
PCR AD0016F/R on modified I716091.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716354
Beta and alpha HbA subunits inserted into the commercial petDUET vector. The vector has each coding sequencing under separate T7 promoters.
false
false
_110_
0
1286
95
It's complicated
false
{{BerkiGEM2007-Biobrick2.0Intro}}
false
Austin Day
BBa_I716354_sequence
1
gaattcatgagatctgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatggtacacttaaccccggaagaaaaatctgcggtgactgctttatggggtaaagttaacgtggatgaagttggtggtgaggcgttgggccgtctgctggttgtgtatccttggacacagcgctttttcgagagctttggtgacctgtctactcccgatgcggtgatgggaaacccgaaagtaaaagcgcatggcaaaaaggtgttgggcgcgttctcggatggtctggcgcatcttgataatcttaaaggtacattcgcgactctgagtgaactccattgtgacaagctccatgttgatcctgagaattttcgtctgctgggtaacgtgctggtgtgtgtccttgcgcatcatttcggtaaggaatttaccccgccggtacaggcggcctatcagaaagtggttgcgggtgtagccaatgcgctggcccataaatatcattaagatttcgagctcggcgcgcctgcaggtcgacaagcttgcggccgcataatgcttaagtcgaacagaaagtaatcgtattgtacacggccgcataatcgaaattaatacgactcactataggggaattgtgagcggataacaattccccatcttagtatattagttaagtataagaaggagatatacatatggttctgtccccagctgataaaacaaacgtaaaagcggcttggggaaaagtcggcgcacacgcgggtgagtatggtgctgaagctttagaacgcatgtttttaagctttcctaccaccaaaacctacttcccgcacttcgatttatcacatgggtcagcacaggtaaagggccacggcaagaaagtagccgacgcgctcaccaacgccgttgcccacgtagacgatatgccgaatgccctgtctgcgctgagcgacctgcatgcgcataaactccgtgtggacccggttaactttaaactgctgtcccactgtctgctggtaaccctcgcggctcatttgccggcagagtttacgccagcggtgcatgcatccctcgataaattcttggcgtcagtgagcacggtgcttaccagcaagtatcgttaaagttctcaattggatatcggccggccacgcgatcgctgacgtcggtacccttgagtctggtaaagaaaccgctgctgcgaaatttgaacgccagcacatggactcgtctactagcgcagcttaattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgggatccttactcgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z