BBa_I716356 1 BBa_I716356 Biobricked Mutant Hemoglobin Cassette in petDUET format 2007-10-23T11:00:00Z 2015-08-31T04:07:52Z Pre-biobricking modifications to I716093. Quickchange AD0013F/R to get rid of EcoR1 site. Quickchange AD0014/F/R to get rid of BglII site. Quickchange AD0015F/R to get rid of XhoI site. PCR AD0016F/R on modified I716093. Digest Bgl2/Xho1 and insert into 9145 plasmid. Product is I716356 Mutant hemoglobins alpha and beta inserted into the commercial petDUET vector. Mutant beta has providence (Asn108Lys) and presbyterian (Lys82Asp) mutantions. Alpha subunit is made into a Di-alpha by a glycine linker. false false _110_ 0 1286 95 No part sequence false {{BerkiGEM2007-Biobrick2.0Intro}} false Austin Day igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z