BBa_I716377 1 BBa_I716377 Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with cytochromes 2007-10-24T11:00:00Z 2015-08-31T04:07:52Z Digest I716375 (BamHI/XhoI, 8218/9, Larger)<br> Digest I716019 (BglII/XhoI, 2063/1401, Smaller)<br> 1,2,3 Product is I716377<br> This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits. The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD. The third part consists of the genes which encode for human cytochrome b5 and human cytochrome b5 reductase. false false _110_ 0 1286 95 No part sequence false {{BerkiGEM2007-Biobrick2.0Intro}} false Austin Day igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z