BBa_I717000 1 BBa_I717000 acGFP<-Pr(lambda)->dsRED 2007-10-02T11:00:00Z 2015-08-31T04:07:52Z AcGFP and dsRED are commercial genes with commercial sequences. The lambda sequence was obtained from literature. This part is a reporter containing the lambda promoter Pr from the lambda switch flanked by a fluorescent reporter gene on each side. DsRED is on the side of Pr promoted by the protein cI, and acGFP is on the side promoted by Cro. An E. coli cell containing this plasmid and infected with lambda phage will fluoresce green if the lytic pathway is chosen or red if the cell becomes lysogenic. false false _159_ 0 1873 9 Not in stock false During design we had to account for differing strengths of RBS in either fluorescent protein gene, since we wanted both genes to be expressed equally. We also had to modify the bistable switch from lambda so that it was less cumbersome. false Blair Lyons annotation1945332 1 Pr from lambda switch range1945332 1 702 823 annotation1945331 1 dsRED range1945331 1 22 701 annotation1945330 1 acGFP range1945330 1 824 1544 BBa_I717000_sequence 1 gaattcgcggccgcttctagagctactgggagccggagtggcgggcctcggcgtgctcgtactgctccaccacggtgtagtcctcgttgtggttggtgatgtccagcttggagtccacgtagtggttgccgggcagctgcacgggcttcttggccttgtacacggtcttgaagtcgcaggtgtagtggccgccgtccttcagcttcagggcgtgggagatctcgcccttcagcacgccgtcctgggggtacagcttctcggtggagggctcccagccggcagtcttcttctgcattacggggccgtcggcggggaagttcacgcccttgaacttcaccttgtagatgaaggtgccgtcctgcagggaggagtcctgctgcacctccaccacgccgccgtcctcgaagttcatggagcgctcccaggtgaagccctcggggaaggacagcttcatgtagtcggggatgtcggcggggtgcttcacgtaggccttggagccgtactggaactggggggacaggatgtcccaggcgaagggcagggggccgcccttggtcacctgcagcttggcggtctgggtgccctcgtagggcttgccctcgccctcgccctcgatctcgaagtagtggccgttcacggagccctccatgcgcaccttgaactgcatgaactccttgatgacgtcctcggtgttgtccatgaacaacctccttcagctcatacgttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttgtccatggtgagcaagggcgccgagctgttcaccggcatcgtgcccatcctgatcgagctgaatggcgatgtgaatggccacaagttcagcgtgagcggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcctgtgccctggcccaccctggtgaccaccctgagctacggcgtgcagtgcttctcacgctaccccgatcacatgaagcagcacgacttcttcaagagcgccatgcctgagggctacatccaggagcgcaccatcttcttcgaggatgacggcaactacaagtcgcgcgccgaggtgaagttcgagggcgataccctggtgaatcgcatcgagctgaccggcaccgatttcaaggaggatggcaacatcctgggcaataagatggagtacaactacaacgcccacaatgtgtacatcatgaccgacaaggccaagaatggcatcaaggtgaacttcaagatccgccacaacatcgaggatggcagcgtgcagctggccgaccactaccagcagaatacccccatcggcgatggccctgtgctgctgcccgataaccactacctgtccacccagagcgccctgtccaaggaccccaacgagaagcgcgatcacatgatctacttcggcttcgtgaccgccgccgccatcacccacggcatggatgagctgtacaagtgatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z