LOCUS BBa_I718001 1015 bp DNA linear 25-Nov-2024 DEFINITION ech My new test generator part Feruloyl CoA hydratase for vanilin ACCESSION BBa_I718001 VERSION BBa_I718001.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..912 /label=ech CDS 82..912 /label=BBa_I718000 stem_loop 928..947 /label=T7 TE misc_feature 954..954 /label=stop polyA_site 948..961 /label=polya misc_feature 921..961 /label=BBa_B0012 terminator 921..961 /label=BBa_B0012 stem_loop 982..1004 /label=stem_loop misc_feature 970..1015 /label=BBa_B0011 terminator 970..1015 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaac tacgaaggtc gctggacaac ggtcaaggtc 121 gaaatcgaag acggcattgc ctgggtcatt ctcaatcgtc cggaaaaacg caacgccatg 181 agcccaaccc tgaaccggga aatgatcgac gtgctggaaa ccctggaaca ggacccggcc 241 gctggtgtgc tggtgctgac cggcgccggc gaggcctgga ccgccggcat ggacctcaag 301 gaatacttcc gcgaggtgga tgccgggccg gagatccttc aggagaaaat ccgccgcgaa 361 gcctcccagt ggcagtggaa actgctgcgc atgtacgcca agccgaccat cgccatggtc 421 aacggctggt gcttcggtgg cggcttcagc ccgctggtgg cgtgcgatct ggcgatctgc 481 gccgacgagg ccaccttcgg cctgtctgaa atcaactggg gcatcccgcc gggcaacctg 541 gtgagcaagg ccatggccga caccgtgggc catcgccagt cgctgtacta catcatgacc 601 ggcaagactt tcggcgggca gaaagccgcc gaaatgggcc tggtcaacga cagcgtgccc 661 ctggctcgat tgcgtgaggt gaccattgag ctggcgcgca acctgctgga gaaaaacccg 721 gtggtgctgc gtgccgccaa gcatggcttc aagcgttgcc gcgagctgac ctgggagcag 781 aacgaagact acctgtacgc caagctcgat cagtcgcgcc tgttggacac cgaaggcggc 841 cgcgagcagg gcatgaagca gtttctcgac gacaagagca tcaagcccgg cctgcaggcg 901 tataaacgct gatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 961 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattt // LOCUS BBa_I760001 1015 bp DNA linear 25-Nov-2024 DEFINITION ech protein generator for vanillin biosynthesis ACCESSION BBa_I760001 VERSION BBa_I760001.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..912 /label=ech (Feruloyl CoA Hydratase) CDS 82..912 /label=BBa_I760000 stem_loop 928..947 /label=T7 TE misc_feature 954..954 /label=stop polyA_site 948..961 /label=polya misc_feature 921..961 /label=BBa_B0012 terminator 921..961 /label=BBa_B0012 stem_loop 982..1004 /label=stem_loop misc_feature 970..1015 /label=BBa_B0011 terminator 970..1015 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaac tacgaaggtc gctggacaac ggtcaaggtc 121 gaaatcgaag acggcattgc ctgggtcatt ctcaatcgtc cggaaaaacg caacgccatg 181 agcccaaccc tgaaccggga aatgatcgac gtgctggaaa ccctggaaca ggacccggcc 241 gctggtgtgc tggtgctgac cggcgccggc gaggcctgga ccgccggcat ggacctcaag 301 gaatacttcc gcgaggtgga tgccgggccg gagatccttc aggagaaaat ccgccgcgaa 361 gcctcccagt ggcagtggaa actgctgcgc atgtacgcca agccgaccat cgccatggtc 421 aacggctggt gcttcggtgg cggcttcagc ccgctggtgg cgtgcgatct ggcgatctgc 481 gccgacgagg ccaccttcgg cctgtctgaa atcaactggg gcatcccgcc gggcaacctg 541 gtgagcaagg ccatggccga caccgtgggc catcgccagt cgctgtacta catcatgacc 601 ggcaagactt tcggcgggca gaaagccgcc gaaatgggcc tggtcaacga cagcgtgccc 661 ctggctcgat tgcgtgaggt gaccattgag ctggcgcgca acctgctgga gaaaaacccg 721 gtggtgctgc gtgccgccaa gcatggcttc aagcgttgcc gcgagctgac ctgggagcag 781 aacgaagact acctgtacgc caagctcgat cagtcgcgcc tgttggacac cgaaggcggc 841 cgcgagcagg gcatgaagca gtttctcgac gacaagagca tcaagcccgg cctgcaggcg 901 tataaacgct gatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 961 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattt // LOCUS BBa_I769104 831 bp DNA linear 25-Nov-2024 DEFINITION Feruloyl CoA hydratase for vanillin biosythesis ACCESSION BBa_I769104 VERSION BBa_I769104.1 FEATURES Location/Qualifiers CDS 1..831 /label=Feruloyl CoA hydratase (ech) ORIGIN 1 atgagcaact acgaaggtcg ctggacaacg gtcaaggtcg aaatcgaaga cggcattgcc 61 tgggtcattc tcaatcgtcc ggaaaaacgc aacgccatga gcccaaccct gaaccgggaa 121 atgatcgacg tgctggaaac cctggaacag gacccggccg ctggtgtgct ggtgctgacc 181 ggcgccggcg aggcctggac cgccggcatg gacctcaagg aatacttccg cgaggtggat 241 gccgggccgg agatccttca ggagaaaatc cgccgcgaag cctcccagtg gcagtggaaa 301 ctgctgcgca tgtacgccaa gccgaccatc gccatggtca acggctggtg cttcggtggc 361 ggcttcagcc cgctggtggc gtgcgatctg gcgatctgcg ccgacgaggc caccttcggc 421 ctgtctgaaa tcaactgggg catcccgccg ggcaacctgg tgagcaaggc catggccgac 481 accgtgggcc atcgccagtc gctgtactac atcatgaccg gcaagacttt cggcgggcag 541 aaagccgccg aaatgggcct ggtcaacgac agcgtgcccc tggctcgatt gcgtgaggtg 601 accattgagc tggcgcgcaa cctgctggag aaaaacccgg tggtgctgcg tgccgccaag 661 catggcttca agcgttgccg cgagctgacc tgggagcaga acgaagacta cctgtacgcc 721 aagctcgatc agtcgcgcct gttggacacc gaaggcggcc gcgagcaggg catgaagcag 781 tttctcgacg acaagagcat caagcccggc ctgcaggcgt ataaacgctg a //