BBa_I718001
type: ComponentDefinition
persistent identity: BBa I718001
display id: BBa_I718001
version: 1
was generated by: iGEM to SBOL conversion
description: ech My new test generator part Feruloyl CoA hydratase for vanilin
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: Uses <partinfo>BBa_I71800</partinfo> as well as a promoter, RBS, and terminator.
owned by: james
owned by: myers
mutable description: This is the part I am editing in the teaching session. It helps synthesize vanilin from ferulic acid with the help of fcs.
discontinued: true
dominant: false
group u list: _141_
m user id: 0
owner id: 1613
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Needs to be used in conjunction with fcs in order to generate vanilin. It uses an ITPG promoter and an high efficiency RBS terminator.
star: false
is replaced by: BBa_I760001
creator: Samuel Bottani
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_I718000
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_I718000
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I718001_sequence/1

BBa_B0011
type: Component
persistent identity: component1935821
display id: component1935821
version: 1
top level: BBa_I718001
definition: BBa_B0011
access: public

BBa_B0034
type: Component
persistent identity: component1935814
display id: component1935814
version: 1
top level: BBa_I718001
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component1935808
display id: component1935808
version: 1
top level: BBa_I718001
definition: lacI+pL
access: public

BBa_B0012
type: Component
persistent identity: component1935817
display id: component1935817
version: 1
top level: BBa_I718001
definition: BBa_B0012
access: public

BBa_I718000
type: Component
persistent identity: component1935816
display id: component1935816
version: 1
top level: BBa_I718001
definition: BBa_I718000
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935808
display id: annotation1935808
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935808/range1935808/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I718001/annotation1935808/range1935808/1
type: Range
persistent identity: range1935808
display id: range1935808
version: 1
top level: BBa_I718001
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935814
display id: annotation1935814
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935814/range1935814/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I718001/annotation1935814/range1935814/1
type: Range
persistent identity: range1935814
display id: range1935814
version: 1
top level: BBa_I718001
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_I718000
type: SequenceAnnotation
persistent identity: annotation1935816
display id: annotation1935816
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935816/range1935816/1
component: BBa_I718000

https://synbiohub.org/public/igem/BBa_I718001/annotation1935816/range1935816/1
type: Range
persistent identity: range1935816
display id: range1935816
version: 1
top level: BBa_I718001
direction: direction/reverse
start: 82
end: 912
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935817
display id: annotation1935817
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935817/range1935817/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718001/annotation1935817/range1935817/1
type: Range
persistent identity: range1935817
display id: range1935817
version: 1
top level: BBa_I718001
direction: direction/forward
start: 921
end: 961
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935821
display id: annotation1935821
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935821/range1935821/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I718001/annotation1935821/range1935821/1
type: Range
persistent identity: range1935821
display id: range1935821
version: 1
top level: BBa_I718001
direction: direction/forward
start: 970
end: 1015
orientation: inline

BBa_I760001
type: ComponentDefinition
persistent identity: BBa I760001
display id: BBa_I760001
version: 1
was generated by: iGEM to SBOL conversion
description: ech protein generator for vanillin biosynthesis
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: uses <partinfo>BBa_I760000</partinfo>, promotor, RBS and terminator. Promotor is EPTG inducible. Contains high efficiency RBS and terminator.
owned by: james
owned by: myers
mutable description: This is a part, that helps to synthesize vanillin from ferulic acid with the help of fcs
discontinued: false
dominant: true
group u list: _148_
m user id: 0
owner id: 1863
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Needs to be used in conjuction with fcs in order to generate vanillin
star: false
creator: Alexej Skvortsov
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_I760000
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_I760000
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I760001_sequence/1

BBa_B0011
type: Component
persistent identity: component1935725
display id: component1935725
version: 1
top level: BBa_I760001
definition: BBa_B0011
access: public

BBa_B0034
type: Component
persistent identity: component1935718
display id: component1935718
version: 1
top level: BBa_I760001
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component1935712
display id: component1935712
version: 1
top level: BBa_I760001
definition: lacI+pL
access: public

BBa_B0012
type: Component
persistent identity: component1935721
display id: component1935721
version: 1
top level: BBa_I760001
definition: BBa_B0012
access: public

BBa_I760000
type: Component
persistent identity: component1935720
display id: component1935720
version: 1
top level: BBa_I760001
definition: BBa_I760000
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935712
display id: annotation1935712
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935712/range1935712/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I760001/annotation1935712/range1935712/1
type: Range
persistent identity: range1935712
display id: range1935712
version: 1
top level: BBa_I760001
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935718
display id: annotation1935718
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935718/range1935718/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I760001/annotation1935718/range1935718/1
type: Range
persistent identity: range1935718
display id: range1935718
version: 1
top level: BBa_I760001
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_I760000
type: SequenceAnnotation
persistent identity: annotation1935720
display id: annotation1935720
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935720/range1935720/1
component: BBa_I760000

https://synbiohub.org/public/igem/BBa_I760001/annotation1935720/range1935720/1
type: Range
persistent identity: range1935720
display id: range1935720
version: 1
top level: BBa_I760001
direction: direction/reverse
start: 82
end: 912
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935721
display id: annotation1935721
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935721/range1935721/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I760001/annotation1935721/range1935721/1
type: Range
persistent identity: range1935721
display id: range1935721
version: 1
top level: BBa_I760001
direction: direction/forward
start: 921
end: 961
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935725
display id: annotation1935725
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935725/range1935725/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I760001/annotation1935725/range1935725/1
type: Range
persistent identity: range1935725
display id: range1935725
version: 1
top level: BBa_I760001
direction: direction/forward
start: 970
end: 1015
orientation: inline

BBa_I718000
type: ComponentDefinition
persistent identity: BBa I718000
display id: BBa_I718000
version: 1
was generated by: iGEM to SBOL conversion
description: Good scenting vanilin compound
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: Genbank AJ536325. Get from pubmed xxxxxx
owned by: james
owned by: myers
mutable description: Helps to convert A to Vanilin
discontinued: true
dominant: false
group u list: _141_
m user id: 0
owner id: 1613
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: fdsdsf,fslkj
star: false
is replaced by: BBa_I769104
creator: Samuel Bottani
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ech
sequence: https://synbiohub.org/public/igem/BBa_I718000_sequence/1

ech
type: SequenceAnnotation
persistent identity: annotation1935613
display id: annotation1935613
version: 1
top level: BBa_I718000
location: https://synbiohub.org/public/igem/BBa_I718000/annotation1935613/range1935613/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I718000/annotation1935613/range1935613/1
type: Range
persistent identity: range1935613
display id: range1935613
version: 1
top level: BBa_I718000
direction: direction/forward
start: 1
end: 831
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_I769104
type: ComponentDefinition
persistent identity: BBa I769104
display id: BBa_I769104
version: 1
was generated by: iGEM to SBOL conversion
description: Feruloyl CoA hydratase for vanillin biosythesis
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:10Z
mutable provenance: Genbank AJ536325 Microb Cell Fact. 2007; 6: 13. Published online 2007 April 16. doi: 10.1186/1475-2859-6-13. Copyright ?? 2007 Barghini et al; licensee BioMed Central Ltd. Vanillin production using metabolically engineered Escherichia coli under non-growing conditions Paolo Barghini,1 Diana Di Gioia,2 Fabio Fava,2 and Maurizio Ruzzicorresponding author1
owned by: james
owned by: myers
mutable description: One of two steps in the bioconversion of freulic acid to vanillin. Comes from Pseudomonas flourescens and works in E. coli. Biosynthesis requires both ech and fcs.
discontinued: false
dominant: true
group u list: _162_
m user id: 0
owner id: 457
owning group id: 67
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Needs fcs and ech
star: false
creator: Meagan Lizarazo
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Feruloyl CoA hydratase (ech)
sequence: https://synbiohub.org/public/igem/BBa_I769104_sequence/1

Feruloyl CoA hydratase (ech)
type: SequenceAnnotation
persistent identity: annotation1935603
display id: annotation1935603
version: 1
top level: BBa_I769104
location: https://synbiohub.org/public/igem/BBa_I769104/annotation1935603/range1935603/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I769104/annotation1935603/range1935603/1
type: Range
persistent identity: range1935603
display id: range1935603
version: 1
top level: BBa_I769104
direction: direction/forward
start: 1
end: 831
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_I760000
type: ComponentDefinition
persistent identity: BBa I760000
display id: BBa_I760000
version: 1
was generated by: iGEM to SBOL conversion
description: feruloyl-CoA hydratase
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: AJ536325. Reports Pseudomonas fluor...[gi:40788116]
owned by: james
owned by: myers
mutable description: feruloyl-CoA hydratase Pseudomonas fluorescens
discontinued: false
dominant: true
group u list: _148_
m user id: 0
owner id: 1863
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: for producing vanillin in E.coli
star: false
creator: Alexej Skvortsov
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ech (Feruloyl CoA Hydratase)
sequence: https://synbiohub.org/public/igem/BBa_I760000_sequence/1

ech (Feruloyl CoA Hydratase)
type: SequenceAnnotation
persistent identity: annotation1935614
display id: annotation1935614
version: 1
top level: BBa_I760000
location: https://synbiohub.org/public/igem/BBa_I760000/annotation1935614/range1935614/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I760000/annotation1935614/range1935614/1
type: Range
persistent identity: range1935614
display id: range1935614
version: 1
top level: BBa_I760000
direction: direction/forward
start: 1
end: 831
orientation: inline

https://synbiohub.org/public/igem/BBa_I718001_sequence/1
type: Sequence
persistent identity: BBa I718001 sequence
display id: BBa_I718001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I760001_sequence/1
type: Sequence
persistent identity: BBa I760001 sequence
display id: BBa_I760001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I718000_sequence/1
type: Sequence
persistent identity: BBa I718000 sequence
display id: BBa_I718000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I769104_sequence/1
type: Sequence
persistent identity: BBa I769104 sequence
display id: BBa_I769104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I760000_sequence/1
type: Sequence
persistent identity: BBa I760000 sequence
display id: BBa_I760000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z