BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_I718011
1
BBa_I718011
rbs-dapA coli
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
none
Released HQ 2013
none
false
false
_141_
0
1481
9
In stock
true
none
false
David Bikard
component1956162
1
BBa_I718004
component1956157
1
BBa_B0030
annotation1956162
1
BBa_I718004
range1956162
1
22
903
annotation1956157
1
BBa_B0030
range1956157
1
1
15
BBa_I718004
1
BBa_I718004
DapA Coli MG1655
2007-07-22T11:00:00Z
2015-08-31T04:07:52Z
E. Coli MG1655
Dihydrodipicolinate synthase is the first enzyme unique to lysine biosynthesis, catalyzing the condensation of pyruvate and aspartate β-semialdehyde by a ping-pong mechanism. It also catalyzes the rate-limiting step in E.coli lysine biosynthesis after aspartate kinase III. The condensing enzyme-pyruvate complex, prior to reduction and hydrolysis, exists as the Schiff base formed between the carbonyl of pyruvate and an ε-amino group of a lysine residue on the enzyme. This property classifies the enzyme as a Class I aldolase with respect to carbon-carbon bond formation. The enzyme is inhibited by lysine, a feedback inhibitor. (source: Ecocyc)
false
false
_141_
0
1481
9
In stock
false
none
false
David Bikard
annotation1939201
1
start
range1939201
1
1
3
annotation1939202
1
stop
range1939202
1
877
882
annotation1939203
1
DapA
range1939203
1
1
882
BBa_I718004_sequence
1
atgttcacgggaagtattgtcgcgattgttactccgatggatgaaaaaggtaatgtctgtcgggctagcttgaaaaaactgattgattatcatgtcgccagcggtacttcggcgatcgtttctgttggcaccactggcgagtccgctaccttaaatcatgacgaacatgctgatgtggtgatgatgacgctggatctggctgatgggcgcattccggtaattgccgggaccggcgctaacgctactgcggaagccattagcctgacgcagcgcttcaatgacagtggtatcgtcggctgcctgacggtaaccccttactacaatcgtccgtcgcaagaaggtttgtatcagcatttcaaagccatcgctgagcatactgacctgccgcaaattctgtataatgtgccgtcccgtactggctgcgatctgctcccggaaacggtgggccgtctggcgaaagtaaaaaatattatcggaatcaaagaggcaacagggaacttaacgcgtgtaaaccagatcaaagagctggtttcagatgattttgttctgctgagcggcgatgatgcgagcgcgctggacttcatgcaattgggcggtcatggggttatttccgttacggctaacgtcgcagcgcgtgatatggcccagatgtgcaaactggcagcagaagggcattttgccgaggcacgcgttattaatcagcgtctgatgccattacacaacaaactatttgtcgaacccaatccaatcccggtgaaatgggcatgtaaggaactgggtcttgtggcgaccgatacgctgcgcctgccaatgacaccaatcaccgacagtggtcgtgagacggtcagagcggcgcttaagcatgccggtttgctgtaataa
BBa_I718011_sequence
1
attaaagaggagaaatactagatgttcacgggaagtattgtcgcgattgttactccgatggatgaaaaaggtaatgtctgtcgggctagcttgaaaaaactgattgattatcatgtcgccagcggtacttcggcgatcgtttctgttggcaccactggcgagtccgctaccttaaatcatgacgaacatgctgatgtggtgatgatgacgctggatctggctgatgggcgcattccggtaattgccgggaccggcgctaacgctactgcggaagccattagcctgacgcagcgcttcaatgacagtggtatcgtcggctgcctgacggtaaccccttactacaatcgtccgtcgcaagaaggtttgtatcagcatttcaaagccatcgctgagcatactgacctgccgcaaattctgtataatgtgccgtcccgtactggctgcgatctgctcccggaaacggtgggccgtctggcgaaagtaaaaaatattatcggaatcaaagaggcaacagggaacttaacgcgtgtaaaccagatcaaagagctggtttcagatgattttgttctgctgagcggcgatgatgcgagcgcgctggacttcatgcaattgggcggtcatggggttatttccgttacggctaacgtcgcagcgcgtgatatggcccagatgtgcaaactggcagcagaagggcattttgccgaggcacgcgttattaatcagcgtctgatgccattacacaacaaactatttgtcgaacccaatccaatcccggtgaaatgggcatgtaaggaactgggtcttgtggcgaccgatacgctgcgcctgccaatgacaccaatcaccgacagtggtcgtgagacggtcagagcggcgcttaagcatgccggtttgctgtaataa
BBa_B0030_sequence
1
attaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z