LOCUS       BBa_I718015             1076 bp    DNA     linear       22-Nov-2024
DEFINITION  ter-lox66-rbs-dapA (B. Subtilis)
ACCESSION   BBa_I718015
VERSION     BBa_I718015.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..171
                     /label=BBa_I718016
     RBS             180..194
                     /label=RBS-1\Strong
     RBS             187..191
                     /label=RBS
     misc_feature    180..194
                     /label=BBa_B0030
     RBS             180..194
                     /label=BBa_B0030
     misc_feature    201..203
                     /label=start
     misc_feature    1071..1076
                     /label=stop
     CDS             201..1076
                     /label=DapA B. Subtilis
     CDS             201..1076
                     /label=BBa_I718005
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagata acttggtata gcatacatta tacgaacggt atactagaga
      181 ttaaagagga gaaatactag atgaatttcg gaaatgtgtc tacagcgatg attacaccct
      241 ttgacaataa agggaacgta gactttcaaa aactgtctac actgattgat tacttgttga
      301 aaaacggaac ggattcttta gtagtagcgg gaacaactgg agaatctccg accctttcaa
      361 ctgaagaaaa aattgcgctt tttgaatata cggtaaaaga agtaaacgga cgggtgcccg
      421 ttatcgctgg tactgggagc aacaacacga aagattccat taagctgaca aaaaaagctg
      481 aggaagcggg cgtggacgct gttatgcttg ttaccccgta ttacaataaa ccttctcaag
      541 aaggaatgta ccagcatttt aaagcaattg cggcagagac atctcttccg gttatgctct
      601 ataatgttcc tggccgtacg gttgcttctc ttgctcctga aacgacgatc cgtttggcgg
      661 cagacattcc gaatgtggtt gcgattaaag aagcgagcgg agacctcgaa gcgattacaa
      721 aaattattgc tgaaacacct gaagacttct atgtctattc aggggatgat gctctaacgc
      781 ttccaattct ttcagttgga ggtagaggag ttgtgtcagt ggcgagccat attgcaggca
      841 ctgatatgca gcaaatgatc aaaaattata cgaatgggca aacagctaat gcggcactga
      901 ttcatcagaa actgctgccg attatgaagg aactgtttaa agcgcctaat cctgctccag
      961 tcaaaacagc gcttcagctg agaggtcttg atgtcggttc tgtgcgtctg cctctagtcc
     1021 cattaactga ggatgaacgt ctgagcttaa gcagcacgat cagcgaactg taataa
//