LOCUS BBa_I718019 4158 bp DNA linear 25-Nov-2024 DEFINITION araC-pBad-mRFP ACCESSION BBa_I718019 VERSION BBa_I718019.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=EcoRI misc_feature 16..21 /label=XbaI CDS 23..952 /label=AraC misc_feature 931..946 /label=ara O2 regulatory region misc_feature 1130..1143 /label=CAP_BS misc_feature 1140..1178 /label=AraI1I2 promoter 1175..1203 /label=ara promoter CDS 1259..1936 /label=mRFP1 stem_loop 1984..2027 /label=rrnB T1 terminator misc_feature 2117..2122 /label=PstI misc_feature 2284..2903 /label=pBR322 replication origin CDS 3058..3918 /label=beta-lactamase ampicillin resistance marker promoter 3960..3988 /label=amp promoter ORIGIN 1 gaattcgcgg ccgcttctag agttatgaca acttgacggc tacatcattc actttttctt 61 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga 121 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg 181 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa 241 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg 301 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct 361 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca 421 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc 481 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc 541 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc 601 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt 661 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc 721 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc 781 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg 841 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca 901 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt 961 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta 1021 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct 1081 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca 1141 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta 1201 ctgtttctcc atacccgttt ttttgggcta gctactagtg aaagaggaga aatactagat 1261 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc 1321 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac 1381 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct 1441 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga 1501 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga 1561 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa 1621 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat 1681 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat 1741 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta 1801 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat 1861 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca 1921 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat 1981 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2041 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2101 atactagaag cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg 2161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 2221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 2281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 2341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 2401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 2461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 2521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 2581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 2641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 2701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 2761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 2821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 2881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 2941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 3061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 3121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 3181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 3301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 3361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 3421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 3481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 3541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 3601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 3661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 3721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 3781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 3841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 3901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 3961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 4081 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt 4141 cgctaaggat gatttctg //