LOCUS       BBa_I718020             3972 bp    DNA     linear       22-Dec-2024
DEFINITION  ptet-lox71-rbs-gfp-ter-lox66-mRFP
ACCESSION   BBa_I718020
VERSION     BBa_I718020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcoRI
     CDS             148..864
                     /label=GFP reporter
     stem_loop       887..930
                     /label=rrnB T1 terminator
     CDS             1073..1750
                     /label=mRFP1
     stem_loop       1798..1841
                     /label=rrnB T1 terminator
     misc_feature    2098..2717
                     /label=pBR322 replication origin
     CDS             2872..3732
                     /label=beta-lactamase ampicillin resistance marker
     promoter        3774..3802
                     /label=amp promoter
     misc_feature    16..21
                     /label=XbaI
     misc_feature    1931..1936
                     /label=PstI
ORIGIN
        1 gaattcgcgg ccgcatctag agtccctatc agtgatagag attgacatcc ctatcagtga
       61 tagagatact gagcactact agagtaccgt tcgtatacga tacattatac gaagttatta
      121 ctagagatta aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
      181 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      241 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      301 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      361 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      421 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      481 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      541 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      601 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      661 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      721 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      781 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      841 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agataacttg
     1021 gtatagcata cattatacga acggtatact agtgaaagag gagaaatact agatggcttc
     1081 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
     1141 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
     1201 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
     1261 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1321 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1381 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1441 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1501 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1561 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1621 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     1681 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     1741 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1861 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1921 gaagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2521 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3841 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3901 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     3961 ggatgatttc tg
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