LOCUS       BBa_I719022              932 bp    DNA     linear       25-Nov-2024
DEFINITION  cI promoter with GFP reporter
ACCESSION   BBa_I719022
VERSION     BBa_I719022.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta ta
//
LOCUS       BBa_S03335               932 bp    DNA     linear       25-Nov-2024
DEFINITION  Pr with GFP as a reporter
ACCESSION   BBa_S03335
VERSION     BBa_S03335.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta ta
//