LOCUS       BBa_I721005             3090 bp    DNA     linear       03-Apr-2025
DEFINITION  pTet>ERBS>AraC>ERBS>LacI>ERBS>ECFP+AAV>Terminator
ACCESSION   BBa_I721005
VERSION     BBa_I721005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..956
                     /label=araC
     misc_feature    957..989
                     /label=LVA
     misc_feature    996..1020
                     /label=Help:Barcodes
     CDS             81..1020
                     /label=BBa_C0080
     misc_feature    63..1020
                     /label=BBa_S03519
     misc_feature    1033..1036
                     /label=conserved
     RBS             1029..1040
                     /label=BBa_B0034
     misc_feature    2136..2174
                     /label=LVA
     CDS             1047..2174
                     /label=lacI-LVA
     misc_feature    1047..2174
                     /label=BBa_C0012
     misc_feature    2175..2199
                     /label=Help:Barcodes
     CDS             1047..2174
                     /label=BBa_C0012
     misc_feature    1029..2199
                     /label=BBa_S03520
     misc_feature    2212..2215
                     /label=conserved
     RBS             2208..2219
                     /label=BBa_B0034
     misc_feature    2226..2987
                     /label=BBa_E0024
     stem_loop       3003..3022
                     /label=T7 TE
     misc_feature    3029..3029
                     /label=stop
     polyA_site      3023..3036
                     /label=polya
     misc_feature    2996..3036
                     /label=BBa_B0012
     terminator      2996..3036
                     /label=BBa_B0012
     stem_loop       3057..3079
                     /label=stem_loop
     misc_feature    3045..3090
                     /label=BBa_B0011
     terminator      3045..3090
                     /label=BBa_B0011
     terminator      2996..3090
                     /label=BBa_B0014
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact
      121 cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt
      181 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc
      241 agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt
      301 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc
      361 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat
      421 ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt
      481 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa
      541 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac
      601 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca
      661 attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc
      721 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc
      781 aggcgaagct gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg
      841 gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga
      901 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcagctg
      961 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     1021 tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg
     1081 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1141 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1201 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1261 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1321 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     1381 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     1441 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     1501 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     1561 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     1621 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     1681 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     1741 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     1801 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     1861 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     1921 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     1981 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     2041 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2101 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt
     2161 tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat
     2221 actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2281 tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2341 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2401 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca
     2461 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2521 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2581 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2641 ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga
     2701 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2761 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2821 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2881 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2941 agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac tagagtcaca
     3001 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc
     3061 cagattatta atccggcttt tttattattt
//