##gff-version 3 ##sequence-region BBa_I721005 1 3090 BBa_I721005 . engineered_region 63 1020 . + 0 ID=BBa_S03519;Name=BBa_S03519 BBa_I721005 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03519 BBa_I721005 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721005 . CDS 81 1020 . + 0 ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03519 BBa_I721005 . engineered_tag 996 1020 . + 0 ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080 BBa_I721005 . tag 957 989 . + 0 ID=annotation308196;Name=LVA;Parent=BBa_C0080 BBa_I721005 . CDS 81 956 . + 0 ID=annotation308191;Name=araC;Parent=BBa_C0080 BBa_I721005 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I721005 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I721005 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I721005 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I721005 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I721005 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I721005 . engineered_region 2226 2987 . + 0 ID=BBa_E0024;Name=BBa_E0024 BBa_I721005 . ribosome_entry_site 2208 2219 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I721005 . conserved_region 2212 2215 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721005 . terminator 2996 3090 . + 0 ID=BBa_B0014;Name=BBa_B0014 BBa_I721005 . terminator 3045 3090 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_I721005 . engineered_region 3045 3090 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_I721005 . stem_loop 3057 3079 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_I721005 . terminator 2996 3036 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_I721005 . polyA_site 3023 3036 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I721005 . stem_loop 3003 3022 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I721005 . engineered_region 2996 3036 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I721005 . stop_codon 3029 3029 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I721005 . engineered_region 1029 2199 . + 0 ID=BBa_S03520;Name=BBa_S03520 BBa_I721005 . CDS 1047 2174 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03520 BBa_I721005 . engineered_region 1047 2174 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_I721005 . CDS 1047 2174 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_I721005 . engineered_tag 2175 2199 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_I721005 . tag 2136 2174 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_I721005 . ribosome_entry_site 1029 1040 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03520 BBa_I721005 . conserved_region 1033 1036 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_I721005 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgatt gaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcg gtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagat tcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcat gaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcg acctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagca attgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcag tacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtc tgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagct gcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcga gtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtca agttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga gaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttat cagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgg agctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccag tctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtg tcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctga tcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttga tgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggc ataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatg cgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcat gttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaat acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc aggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaaga ggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgac gtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatct gcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgcta ccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttc aaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagg gcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcac cgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgcc gaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccg ccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcgg catggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagtcacact ggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctttt ttattattt