##gff-version 3
##sequence-region BBa_I721006 1 2748
BBa_I721006	.	engineered_region	1884	2645	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_I721006	.	tag	2601	2639	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I721006	.	stop_codon	2640	2645	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I721006	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I721006	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I721006	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I721006	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I721006	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I721006	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I721006	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I721006	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I721006	.	engineered_region	920	1877	.	+	0	ID=BBa_S03519;Name=BBa_S03519
BBa_I721006	.	CDS	938	1877	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03519
BBa_I721006	.	engineered_tag	1853	1877	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I721006	.	tag	1814	1846	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I721006	.	CDS	938	1813	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I721006	.	ribosome_entry_site	920	931	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03519
BBa_I721006	.	conserved_region	924	927	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I721006	.	terminator	2654	2748	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_I721006	.	terminator	2654	2694	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_I721006	.	polyA_site	2681	2694	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I721006	.	engineered_region	2654	2694	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I721006	.	stop_codon	2687	2687	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I721006	.	stem_loop	2661	2680	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I721006	.	terminator	2703	2748	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_I721006	.	stem_loop	2715	2737	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I721006	.	engineered_region	2703	2748	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I721006	.	engineered_region	209	911	.	+	0	ID=BBa_S03518;Name=BBa_S03518
BBa_I721006	.	CDS	227	911	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_I721006	.	tag	847	880	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I721006	.	CDS	230	846	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I721006	.	engineered_tag	887	911	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I721006	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_I721006	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I721006
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggctgaagc
gcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggt
tatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtgg
tgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacgg
tcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaac
tggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggc
aaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcg
gcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgat
cacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttt
tccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcac
tacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaa
tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagatggtgagcaagg
gcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtc
cggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccc
tggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgact
tcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccg
cgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaac
atcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatca
aggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccat
cggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaag
cgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctg
ctgcaaacgacgaaaactacgctgctgctgtttaataatactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt