LOCUS BBa_I721007 3935 bp DNA linear 25-Nov-2024 DEFINITION pAraC/BAD>ERBS>LacI>ERBS>TetR>ERBS>mRFP1>Terminator ACCESSION BBa_I721007 VERSION BBa_I721007.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 misc_feature 1219..2389 /label=BBa_S03520 misc_feature 2402..2405 /label=conserved RBS 2398..2409 /label=BBa_B0034 CDS 2419..3035 /label=tetR misc_feature 3036..3069 /label=SsrA misc_feature 3076..3100 /label=Help:Barcodes CDS 2416..3100 /label=BBa_C0040 misc_feature 2398..3100 /label=BBa_S03518 misc_feature 3113..3116 /label=conserved RBS 3109..3120 /label=BBa_B0034 CDS 3127..3801 /label=mrfp1 misc_feature 3808..3832 /label=Help:Barcodes CDS 3127..3832 /label=BBa_E1010 stem_loop 3848..3867 /label=T7 TE misc_feature 3874..3874 /label=stop polyA_site 3868..3881 /label=polya misc_feature 3841..3881 /label=BBa_B0012 terminator 3841..3881 /label=BBa_B0012 stem_loop 3902..3924 /label=stem_loop misc_feature 3890..3935 /label=BBa_B0011 terminator 3890..3935 /label=BBa_B0011 terminator 3841..3935 /label=BBa_B0014 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa 2401 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag 2461 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt 2521 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta 2581 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg 2641 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat 2701 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat 2761 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc 2821 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa 2881 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa 2941 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc 3001 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct 3061 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagaa agaggagaaa 3121 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 3181 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 3241 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 3301 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 3361 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 3421 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 3481 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 3541 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 3601 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 3661 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 3721 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 3781 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 3841 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 3901 aaagccagat tattaatccg gcttttttat tattt //