##gff-version 3 ##sequence-region BBa_I721007 1 3935 BBa_I721007 . CDS 3127 3832 . + 0 ID=BBa_E1010;Name=BBa_E1010 BBa_I721007 . CDS 3127 3801 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_I721007 . engineered_tag 3808 3832 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_I721007 . ribosome_entry_site 3109 3120 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I721007 . conserved_region 3113 3116 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721007 . terminator 3841 3935 . + 0 ID=BBa_B0014;Name=BBa_B0014 BBa_I721007 . terminator 3890 3935 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_I721007 . engineered_region 3890 3935 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_I721007 . stem_loop 3902 3924 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_I721007 . terminator 3841 3881 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_I721007 . polyA_site 3868 3881 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I721007 . stem_loop 3848 3867 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I721007 . engineered_region 3841 3881 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I721007 . stop_codon 3874 3874 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I721007 . engineered_region 2398 3100 . + 0 ID=BBa_S03518;Name=BBa_S03518 BBa_I721007 . ribosome_entry_site 2398 2409 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518 BBa_I721007 . conserved_region 2402 2405 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721007 . CDS 2416 3100 . + 0 ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518 BBa_I721007 . CDS 2419 3035 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_I721007 . tag 3036 3069 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_I721007 . engineered_tag 3076 3100 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_I721007 . promoter 1 1210 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_I721007 . promoter 1043 1114 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_I721007 . promoter 1080 1210 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_I721007 . sequence_feature 1 1079 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_I721007 . engineered_region 1219 2389 . + 0 ID=BBa_S03520;Name=BBa_S03520 BBa_I721007 . CDS 1237 2364 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03520 BBa_I721007 . engineered_region 1237 2364 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_I721007 . CDS 1237 2364 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_I721007 . engineered_tag 2365 2389 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_I721007 . tag 2326 2364 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_I721007 . ribosome_entry_site 1219 1230 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03520 BBa_I721007 . conserved_region 1223 1226 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_I721007 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggt agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga tagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaaca gcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagca gcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccat actcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttac taagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatt agcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggt tgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattat tacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgg attagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactga tagtgctagtgtagatcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttca tgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgta cgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcag ttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtt tcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtga gttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgg gaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacg gtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccga catcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcc accggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttc tgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt