LOCUS       BBa_I721008             3012 bp    DNA     linear       15-May-2024
DEFINITION  Dual Luciferase Assay for Promoters (dlap)
ACCESSION   BBa_I721008
VERSION     BBa_I721008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1733
                     /label=BBa_I712019
     stem_loop       1753..1796
                     /label=stem_loop
     misc_feature    1742..1821
                     /label=BBa_B0010
     terminator      1742..1821
                     /label=BBa_B0010
     stem_loop       1837..1856
                     /label=T7 TE
     misc_feature    1863..1863
                     /label=stop
     polyA_site      1857..1870
                     /label=polya
     misc_feature    1830..1870
                     /label=BBa_B0012
     terminator      1830..1870
                     /label=BBa_B0012
     promoter        1879..1913
                     /label=BBa_J23100
     misc_feature    1926..1929
                     /label=conserved
     RBS             1922..1933
                     /label=BBa_B0034
     misc_feature    1940..2875
                     /label=Rluc
     CDS             1940..2875
                     /label=Rluc
     misc_feature    1940..2875
                     /label=BBa_J52008
     stem_loop       2895..2938
                     /label=stem_loop
     misc_feature    2884..2963
                     /label=BBa_B0010
     terminator      2884..2963
                     /label=BBa_B0010
     stem_loop       2979..2998
                     /label=T7 TE
     misc_feature    3005..3005
                     /label=stop
     polyA_site      2999..3012
                     /label=polya
     misc_feature    2972..3012
                     /label=BBa_B0012
     terminator      2972..3012
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
      121 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
      181 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
      241 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
      301 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
      361 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
      421 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
      481 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
      541 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
      601 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
      661 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
      721 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
      781 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
      841 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
      901 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
      961 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     1021 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     1081 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     1141 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     1201 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     1261 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     1321 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     1381 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     1441 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     1501 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     1561 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     1621 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     1681 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taatactaga
     1741 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1801 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1861 tgcgtttata tactagagtt gacggctagc tcagtcctag gtacagtgct agctactaga
     1921 gaaagaggag aaatactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
     1981 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
     2041 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
     2101 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
     2161 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
     2221 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
     2281 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
     2341 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
     2401 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
     2461 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
     2521 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
     2581 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
     2641 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
     2701 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
     2761 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
     2821 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
     2881 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2941 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3001 tctgcgttta ta
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