BBa_I721008
type: ComponentDefinition
persistent identity: BBa I721008
display id: BBa_I721008
version: 1
was generated by: iGEM to SBOL conversion
description: Dual Luciferase Assay for Promoters (dlap)
created: 2008-02-27T12:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: registry parts
owned by: james
owned by: myers
mutable description: dlap assay
discontinued: false
dominant: false
group u list: _113_
m user id: 0
owner id: 604
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Experimental promoter is added before the firefly luciferase, control promoter is added before the renilla luciferase gene.
star: false
creator: John Cumbers
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J23100
component: BBa_B0012
component: BBa_J52008
component: BBa_B0034
component: BBa_R0040
component: BBa_B0010
component: BBa_I712019
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I712019
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence annotation: BBa_J52008
sequence: https://synbiohub.org/public/igem/BBa_I721008_sequence/1

BBa_J23100
type: Component
persistent identity: component1961263
display id: component1961263
version: 1
top level: BBa_I721008
definition: BBa_J23100
access: public

BBa_B0012
type: Component
persistent identity: component1961259
display id: component1961259
version: 1
top level: BBa_I721008
definition: BBa_B0012
access: public

BBa_J52008
type: Component
persistent identity: component1961268
display id: component1961268
version: 1
top level: BBa_I721008
definition: BBa_J52008
access: public

BBa_B0034
type: Component
persistent identity: component1961265
display id: component1961265
version: 1
top level: BBa_I721008
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component1961255
display id: component1961255
version: 1
top level: BBa_I721008
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1961250
display id: component1961250
version: 1
top level: BBa_I721008
definition: p(tetR)
access: public

BBa_B0010
type: Component
persistent identity: component1961257
display id: component1961257
version: 1
top level: BBa_I721008
definition: BBa_B0010
access: public

BBa_I712019
type: Component
persistent identity: component1961256
display id: component1961256
version: 1
top level: BBa_I721008
definition: BBa_I712019
access: public

BBa_B0012
type: Component
persistent identity: component1961271
display id: component1961271
version: 1
top level: BBa_I721008
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1961269
display id: component1961269
version: 1
top level: BBa_I721008
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1961250
display id: annotation1961250
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961250/range1961250/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I721008/annotation1961250/range1961250/1
type: Range
persistent identity: range1961250
display id: range1961250
version: 1
top level: BBa_I721008
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1961255
display id: annotation1961255
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961255/range1961255/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I721008/annotation1961255/range1961255/1
type: Range
persistent identity: range1961255
display id: range1961255
version: 1
top level: BBa_I721008
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation1961256
display id: annotation1961256
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961256/range1961256/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_I721008/annotation1961256/range1961256/1
type: Range
persistent identity: range1961256
display id: range1961256
version: 1
top level: BBa_I721008
direction: direction/reverse
start: 81
end: 1733
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961257
display id: annotation1961257
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961257/range1961257/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I721008/annotation1961257/range1961257/1
type: Range
persistent identity: range1961257
display id: range1961257
version: 1
top level: BBa_I721008
direction: direction/forward
start: 1742
end: 1821
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961259
display id: annotation1961259
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961259/range1961259/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I721008/annotation1961259/range1961259/1
type: Range
persistent identity: range1961259
display id: range1961259
version: 1
top level: BBa_I721008
direction: direction/forward
start: 1830
end: 1870
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1961263
display id: annotation1961263
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961263/range1961263/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_I721008/annotation1961263/range1961263/1
type: Range
persistent identity: range1961263
display id: range1961263
version: 1
top level: BBa_I721008
direction: direction/reverse
start: 1879
end: 1913
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1961265
display id: annotation1961265
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961265/range1961265/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I721008/annotation1961265/range1961265/1
type: Range
persistent identity: range1961265
display id: range1961265
version: 1
top level: BBa_I721008
direction: direction/reverse
start: 1922
end: 1933
orientation: inline

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation1961268
display id: annotation1961268
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961268/range1961268/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_I721008/annotation1961268/range1961268/1
type: Range
persistent identity: range1961268
display id: range1961268
version: 1
top level: BBa_I721008
direction: direction/reverse
start: 1940
end: 2875
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961269
display id: annotation1961269
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961269/range1961269/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I721008/annotation1961269/range1961269/1
type: Range
persistent identity: range1961269
display id: range1961269
version: 1
top level: BBa_I721008
direction: direction/forward
start: 2884
end: 2963
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961271
display id: annotation1961271
version: 1
top level: BBa_I721008
location: https://synbiohub.org/public/igem/BBa_I721008/annotation1961271/range1961271/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I721008/annotation1961271/range1961271/1
type: Range
persistent identity: range1961271
display id: range1961271
version: 1
top level: BBa_I721008
direction: direction/forward
start: 2972
end: 3012
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: iGEM to SBOL conversion
description: Firefly luciferase - luciferase from Photinus pyralis
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part is cloned into pSBAK3 vector with terminator.
star: true
creator: Anja Korenčič
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: iGEM to SBOL conversion
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
star: true
creator: Monika Ciglic
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_I721008_sequence/1
type: Sequence
persistent identity: BBa I721008 sequence
display id: BBa_I721008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712019_sequence/1
type: Sequence
persistent identity: BBa I712019 sequence
display id: BBa_I712019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52008_sequence/1
type: Sequence
persistent identity: BBa J52008 sequence
display id: BBa_J52008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z