LOCUS       BBa_I722004             3805 bp    DNA     linear       14-Nov-2024
DEFINITION  Yiplac211(-EcoRI)
ACCESSION   BBa_I722004
VERSION     BBa_I722004.1  
FEATURES             Location/Qualifiers
     primer_bind     318..334
                     /label=M13/PUC sequencing primer
     primer_bind     204..220
                     /label=M13/PUC reverse sequencing primer
     CDS             785..1585
                     /label=URA3
     CDS             2004..2861
                     /label=AMP
     misc_feature    285..290
                     /label=EcoRI
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattaa ttaattcact
      301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      421 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      481 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      601 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      661 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcgggtaat aactgatata
      721 attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt
      781 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
      841 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
      901 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
      961 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     1021 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     1081 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca
     1141 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa
     1201 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     1261 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     1321 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     1381 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     1441 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     1501 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     1561 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcatgtt tttgttctgt
     1621 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat
     1681 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
     1741 caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
     1801 attgaaaagc tggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1861 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2221 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2521 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3181 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3781 agtcagtgag cgaggaagcg gaaga
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