LOCUS BBa_I722004 3805 bp DNA linear 22-Nov-2024 DEFINITION Yiplac211(-EcoRI) ACCESSION BBa_I722004 VERSION BBa_I722004.1 FEATURES Location/Qualifiers primer_bind 318..334 /label=M13/PUC sequencing primer primer_bind 204..220 /label=M13/PUC reverse sequencing primer CDS 785..1585 /label=URA3 CDS 2004..2861 /label=AMP misc_feature 285..290 /label=EcoRI ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattaa ttaattcact 301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 421 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac 481 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 541 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 601 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 661 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcgggtaat aactgatata 721 attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt 781 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt 841 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt 901 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct 961 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac 1021 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa 1081 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca 1141 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa 1201 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata 1261 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga 1321 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg 1381 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt 1441 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt 1501 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac 1561 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcatgtt tttgttctgt 1621 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat 1681 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat 1741 caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga 1801 attgaaaagc tggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 1861 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 1921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 1981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 2041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 2101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 2161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 2221 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 2281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 2341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 2401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 2461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 2521 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 2581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 2641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 2701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 2761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 2821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 2881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 2941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3181 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 3661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 3721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 3781 agtcagtgag cgaggaagcg gaaga //