LOCUS       BBa_I722005             1864 bp    DNA     linear       17-May-2024
DEFINITION  Inducible AHL and CI generator(with CI promoter)
ACCESSION   BBa_I722005
VERSION     BBa_I722005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    806..837
                     /label=LVA
     CDS             227..805
                     /label=luxI
     misc_feature    227..844
                     /label=BBa_C0061
     misc_feature    845..869
                     /label=Help:Barcodes
     CDS             227..844
                     /label=BBa_C0061
     misc_feature    209..869
                     /label=BBa_C0261
     misc_feature    882..885
                     /label=conserved
     RBS             878..889
                     /label=BBa_B0034
     CDS             899..1606
                     /label=cI lambda
     misc_feature    1607..1639
                     /label=LVA
     misc_feature    1646..1670
                     /label=Help:Barcodes
     CDS             896..1645
                     /label=BBa_C0051
     stem_loop       1690..1733
                     /label=stem_loop
     misc_feature    1679..1758
                     /label=BBa_B0010
     terminator      1679..1758
                     /label=BBa_B0010
     stem_loop       1774..1793
                     /label=T7 TE
     misc_feature    1800..1800
                     /label=stop
     polyA_site      1794..1807
                     /label=polya
     misc_feature    1767..1807
                     /label=BBa_B0012
     terminator      1767..1807
                     /label=BBa_B0012
     promoter        1853..1858
                     /label=-10
     promoter        1830..1835
                     /label=-35
     misc_feature    1840..1856
                     /label=OR1
     misc_feature    1816..1832
                     /label=OR2
     misc_feature    1816..1864
                     /label=BBa_R0051
     promoter        1816..1864
                     /label=BBa_R0051
     misc_feature    878..1864
                     /label=BBa_Q04510
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta
      841 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatgag
      901 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      961 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
     1021 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
     1081 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
     1141 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
     1201 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
     1261 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
     1321 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
     1381 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
     1441 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
     1501 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
     1561 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga
     1621 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc
     1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1801 gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg
     1861 ttgc
//