LOCUS BBa_I722008 3757 bp DNA linear 29-Nov-2024 DEFINITION GFP Producer induced by IPTG ACCESSION BBa_I722008 VERSION BBa_I722008.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 806..837 /label=LVA CDS 227..805 /label=luxI misc_feature 227..844 /label=BBa_C0061 misc_feature 845..869 /label=Help:Barcodes CDS 227..844 /label=BBa_C0061 misc_feature 209..869 /label=BBa_C0261 misc_feature 882..885 /label=conserved RBS 878..889 /label=BBa_B0034 CDS 899..1606 /label=cI lambda misc_feature 1607..1639 /label=LVA misc_feature 1646..1670 /label=Help:Barcodes CDS 896..1645 /label=BBa_C0051 stem_loop 1690..1733 /label=stem_loop misc_feature 1679..1758 /label=BBa_B0010 terminator 1679..1758 /label=BBa_B0010 stem_loop 1774..1793 /label=T7 TE misc_feature 1800..1800 /label=stop polyA_site 1794..1807 /label=polya misc_feature 1767..1807 /label=BBa_B0012 terminator 1767..1807 /label=BBa_B0012 promoter 1853..1858 /label=-10 promoter 1830..1835 /label=-35 misc_feature 1840..1856 /label=OR1 misc_feature 1816..1832 /label=OR2 misc_feature 1816..1864 /label=BBa_R0051 promoter 1816..1864 /label=BBa_R0051 misc_feature 878..1864 /label=BBa_Q04510 misc_feature 1..1864 /label=BBa_I722005 misc_feature 1877..1880 /label=conserved RBS 1873..1884 /label=BBa_B0034 misc_feature 1891..1892 /label=prefix misc_feature 2064..2064 /label=T misc_feature 2382..2382 /label=A CDS 1891..2640 /label=luxR misc_feature 1891..2646 /label=BBa_C0062 misc_feature 2647..2671 /label=Help:Barcodes CDS 1891..2646 /label=BBa_C0062 stem_loop 2691..2734 /label=stem_loop misc_feature 2680..2759 /label=BBa_B0010 terminator 2680..2759 /label=BBa_B0010 stem_loop 2775..2794 /label=T7 TE misc_feature 2801..2801 /label=stop polyA_site 2795..2808 /label=polya misc_feature 2768..2808 /label=BBa_B0012 terminator 2768..2808 /label=BBa_B0012 misc_feature 2817..2836 /label=LuxR/HSL promoter 2836..2841 /label=-35 promoter 2858..2863 /label=-10 misc_feature 2869..2869 /label=start misc_feature 2817..2871 /label=BBa_R0062 promoter 2817..2871 /label=BBa_R0062 misc_feature 1873..2871 /label=BBa_A340620 misc_feature 1..2871 /label=BBa_S03827 RBS 2880..2894 /label=RBS-1\Strong RBS 2887..2891 /label=RBS misc_feature 2880..2894 /label=BBa_B0030 RBS 2880..2894 /label=BBa_B0030 CDS 2901..3620 /label=GFP protein CDS 2901..3620 /label=BBa_E0040 stem_loop 3640..3683 /label=stem_loop misc_feature 3629..3708 /label=BBa_B0010 terminator 3629..3708 /label=BBa_B0010 stem_loop 3724..3743 /label=T7 TE misc_feature 3750..3750 /label=stop polyA_site 3744..3757 /label=polya misc_feature 3717..3757 /label=BBa_B0012 terminator 3717..3757 /label=BBa_B0012 misc_feature 2880..3757 /label=BBa_E0840 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat 241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta 841 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatgag 901 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 961 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 1021 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 1081 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 1141 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 1201 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 1261 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 1321 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 1381 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 1441 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 1501 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 1561 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 1621 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1801 gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg 1861 ttgctactag agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac 1921 agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct 1981 gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct 2041 atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat 2101 gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc caatcattca 2161 ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt 2221 aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac 2281 aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta 2341 tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga 2401 aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt 2461 ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt 2521 gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc 2581 tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat 2641 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa 2701 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2761 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct 2821 gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga 2881 ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 2941 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 3001 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 3061 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 3121 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 3181 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 3241 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 3301 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 3361 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 3421 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 3481 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 3541 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 3601 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3661 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3721 actggctcac cttcgggtgg gcctttctgc gtttata //