##gff-version 3
##sequence-region BBa_I722008 1 3757
BBa_I722008	.	engineered_region	2880	3757	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_I722008	.	ribosome_entry_site	2880	2894	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_I722008	.	engineered_region	2880	2894	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I722008	.	ribosome_entry_site	2880	2894	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I722008	.	ribosome_entry_site	2887	2891	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I722008	.	CDS	2901	3620	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_I722008	.	CDS	2901	3620	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I722008	.	terminator	3629	3708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_I722008	.	stem_loop	3640	3683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I722008	.	engineered_region	3629	3708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I722008	.	terminator	3717	3757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_I722008	.	engineered_region	3717	3757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I722008	.	stop_codon	3750	3750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I722008	.	polyA_site	3744	3757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I722008	.	stem_loop	3724	3743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I722008	.	engineered_region	1	2871	.	+	0	ID=BBa_S03827;Name=BBa_S03827
BBa_I722008	.	engineered_region	1	1864	.	+	0	ID=BBa_I722005;Name=BBa_I722005;Parent=BBa_S03827
BBa_I722008	.	engineered_region	878	1864	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_I722005
BBa_I722008	.	terminator	1767	1807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_I722008	.	engineered_region	1767	1807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I722008	.	stop_codon	1800	1800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I722008	.	polyA_site	1794	1807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I722008	.	stem_loop	1774	1793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I722008	.	promoter	1816	1864	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_I722008	.	engineered_region	1816	1864	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I722008	.	promoter	1830	1835	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I722008	.	promoter	1853	1858	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I722008	.	non_covalent_binding_site	1816	1832	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I722008	.	non_covalent_binding_site	1840	1856	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I722008	.	terminator	1679	1758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_I722008	.	stem_loop	1690	1733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I722008	.	engineered_region	1679	1758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I722008	.	ribosome_entry_site	878	889	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_I722008	.	conserved_region	882	885	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722008	.	CDS	896	1645	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_I722008	.	tag	1607	1639	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I722008	.	engineered_tag	1646	1670	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I722008	.	CDS	899	1606	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I722008	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I722005
BBa_I722008	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I722008	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I722008	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I722008	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I722008	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I722008	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I722008	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I722008	.	engineered_region	209	869	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_I722005
BBa_I722008	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_I722008	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722008	.	CDS	227	844	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_I722008	.	engineered_region	227	844	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I722008	.	engineered_tag	845	869	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I722008	.	tag	806	837	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I722008	.	CDS	227	805	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I722008	.	engineered_region	1873	2871	.	+	0	ID=BBa_A340620;Name=BBa_A340620;Parent=BBa_S03827
BBa_I722008	.	terminator	2680	2759	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_I722008	.	stem_loop	2691	2734	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I722008	.	engineered_region	2680	2759	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I722008	.	ribosome_entry_site	1873	1884	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_I722008	.	conserved_region	1877	1880	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722008	.	CDS	1891	2646	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_I722008	.	point_mutation	2382	2382	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I722008	.	engineered_region	1891	2646	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I722008	.	engineered_region	1891	1892	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I722008	.	CDS	1891	2640	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I722008	.	point_mutation	2064	2064	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I722008	.	engineered_tag	2647	2671	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I722008	.	promoter	2817	2871	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_I722008	.	promoter	2836	2841	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I722008	.	engineered_region	2817	2871	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I722008	.	start_codon	2869	2869	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I722008	.	promoter	2858	2863	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I722008	.	non_covalent_binding_site	2817	2836	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I722008	.	terminator	2768	2808	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_I722008	.	engineered_region	2768	2808	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I722008	.	stop_codon	2801	2801	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I722008	.	polyA_site	2795	2808	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I722008	.	stem_loop	2775	2794	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I722008
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgactataat
gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag
caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt
atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt
ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa
aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc
aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg
tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
aaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcg
ccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatgggg
cagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctca
aagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtc
acttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttacc
aaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaatt
ccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttga
gccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtg
tttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgcta
gtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgcta
gtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagta
acaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgaaa
aacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgct
tatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctga
tatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctata
gtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaa
atgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgg
aatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatta
attgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagag
aaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtac
tgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttta
acaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaa
gaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata