LOCUS BBa_I722009 2750 bp DNA linear 25-Nov-2024 DEFINITION Test of expression of CI repressor ACCESSION BBa_I722009 VERSION BBa_I722009.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 806..837 /label=LVA CDS 227..805 /label=luxI misc_feature 227..844 /label=BBa_C0061 misc_feature 845..869 /label=Help:Barcodes CDS 227..844 /label=BBa_C0061 misc_feature 209..869 /label=BBa_C0261 misc_feature 882..885 /label=conserved RBS 878..889 /label=BBa_B0034 CDS 899..1606 /label=cI lambda misc_feature 1607..1639 /label=LVA misc_feature 1646..1670 /label=Help:Barcodes CDS 896..1645 /label=BBa_C0051 stem_loop 1690..1733 /label=stem_loop misc_feature 1679..1758 /label=BBa_B0010 terminator 1679..1758 /label=BBa_B0010 stem_loop 1774..1793 /label=T7 TE misc_feature 1800..1800 /label=stop polyA_site 1794..1807 /label=polya misc_feature 1767..1807 /label=BBa_B0012 terminator 1767..1807 /label=BBa_B0012 promoter 1853..1858 /label=-10 promoter 1830..1835 /label=-35 misc_feature 1840..1856 /label=OR1 misc_feature 1816..1832 /label=OR2 misc_feature 1816..1864 /label=BBa_R0051 promoter 1816..1864 /label=BBa_R0051 misc_feature 878..1864 /label=BBa_Q04510 misc_feature 1..1864 /label=BBa_I722005 RBS 1873..1887 /label=RBS-1\Strong RBS 1880..1884 /label=RBS misc_feature 1873..1887 /label=BBa_B0030 RBS 1873..1887 /label=BBa_B0030 CDS 1894..2613 /label=GFP protein CDS 1894..2613 /label=BBa_E0040 stem_loop 2633..2676 /label=stem_loop misc_feature 2622..2701 /label=BBa_B0010 terminator 2622..2701 /label=BBa_B0010 stem_loop 2717..2736 /label=T7 TE misc_feature 2743..2743 /label=stop polyA_site 2737..2750 /label=polya misc_feature 2710..2750 /label=BBa_B0012 terminator 2710..2750 /label=BBa_B0012 misc_feature 1873..2750 /label=BBa_E0840 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat 241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta 841 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatgag 901 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 961 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 1021 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 1081 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 1141 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 1201 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 1261 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 1321 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 1381 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 1441 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 1501 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 1561 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 1621 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1801 gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg 1861 ttgctactag agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact 1921 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 1981 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact 2041 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa 2101 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc 2161 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt 2221 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat 2281 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat 2341 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac 2401 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc 2461 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa 2521 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt 2581 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg 2641 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2701 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //