LOCUS BBa_I722010 5135 bp DNA linear 22-Dec-2024 DEFINITION RS Flip-Flop Design ACCESSION BBa_I722010 VERSION BBa_I722010.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 63..1370 /label=BBa_P0412 misc_feature 1..1370 /label=BBa_I722007 CDS 1379..1466 /label=end of LacI coding region (inactive) misc_feature 1379..1578 /label=BBa_R0010 misc_feature 1467..1504 /label=CAP binding site promoter 1515..1520 /label=-35 promoter 1539..1544 /label=-10 misc_feature 1544..1578 /label=LacI binding site misc_feature 1551..1551 /label=start promoter 1379..1578 /label=BBa_R0010 misc_feature 1591..1594 /label=conserved RBS 1587..1598 /label=BBa_B0034 misc_feature 2184..2215 /label=LVA CDS 1605..2183 /label=luxI misc_feature 1605..2222 /label=BBa_C0061 misc_feature 2223..2247 /label=Help:Barcodes CDS 1605..2222 /label=BBa_C0061 misc_feature 1587..2247 /label=BBa_C0261 misc_feature 2260..2263 /label=conserved RBS 2256..2267 /label=BBa_B0034 CDS 2277..2984 /label=cI lambda misc_feature 2985..3017 /label=LVA misc_feature 3024..3048 /label=Help:Barcodes CDS 2274..3023 /label=BBa_C0051 stem_loop 3068..3111 /label=stem_loop misc_feature 3057..3136 /label=BBa_B0010 terminator 3057..3136 /label=BBa_B0010 stem_loop 3152..3171 /label=T7 TE misc_feature 3178..3178 /label=stop polyA_site 3172..3185 /label=polya misc_feature 3145..3185 /label=BBa_B0012 terminator 3145..3185 /label=BBa_B0012 promoter 3231..3236 /label=-10 promoter 3208..3213 /label=-35 misc_feature 3218..3234 /label=OR1 misc_feature 3194..3210 /label=OR2 misc_feature 3194..3242 /label=BBa_R0051 promoter 3194..3242 /label=BBa_R0051 misc_feature 2256..3242 /label=BBa_Q04510 misc_feature 1379..3242 /label=BBa_I722005 misc_feature 3255..3258 /label=conserved RBS 3251..3262 /label=BBa_B0034 misc_feature 3269..3270 /label=prefix misc_feature 3442..3442 /label=T misc_feature 3760..3760 /label=A CDS 3269..4018 /label=luxR misc_feature 3269..4024 /label=BBa_C0062 misc_feature 4025..4049 /label=Help:Barcodes CDS 3269..4024 /label=BBa_C0062 stem_loop 4069..4112 /label=stem_loop misc_feature 4058..4137 /label=BBa_B0010 terminator 4058..4137 /label=BBa_B0010 stem_loop 4153..4172 /label=T7 TE misc_feature 4179..4179 /label=stop polyA_site 4173..4186 /label=polya misc_feature 4146..4186 /label=BBa_B0012 terminator 4146..4186 /label=BBa_B0012 misc_feature 4195..4214 /label=LuxR/HSL promoter 4214..4219 /label=-35 promoter 4236..4241 /label=-10 misc_feature 4247..4247 /label=start misc_feature 4195..4249 /label=BBa_R0062 promoter 4195..4249 /label=BBa_R0062 misc_feature 3251..4249 /label=BBa_A340620 misc_feature 1379..4249 /label=BBa_S03827 RBS 4258..4272 /label=RBS-1\Strong RBS 4265..4269 /label=RBS misc_feature 4258..4272 /label=BBa_B0030 RBS 4258..4272 /label=BBa_B0030 CDS 4279..4998 /label=GFP protein CDS 4279..4998 /label=BBa_E0040 stem_loop 5018..5061 /label=stem_loop misc_feature 5007..5086 /label=BBa_B0010 terminator 5007..5086 /label=BBa_B0010 stem_loop 5102..5121 /label=T7 TE misc_feature 5128..5128 /label=stop polyA_site 5122..5135 /label=polya misc_feature 5095..5135 /label=BBa_B0012 terminator 5095..5135 /label=BBa_B0012 misc_feature 4258..5135 /label=BBa_E0840 misc_feature 1379..5135 /label=BBa_I722008 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca 1381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 1501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 1561 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatgact ataatgataa 1621 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1681 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1741 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1801 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1861 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1921 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1981 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 2041 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 2101 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 2161 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2221 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatgagca 2281 caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt 2341 atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga 2401 tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg 2461 ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca 2521 gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg 2581 agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta 2641 ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct 2701 ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg 2761 acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag 2821 ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt 2881 ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg 2941 tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa 3001 actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag 3061 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3121 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3181 ttatatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt 3241 gctactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag 3301 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga 3361 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat 3421 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga 3481 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc 3541 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa 3601 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa 3661 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt 3721 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa 3781 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt 3841 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga 3901 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg 3961 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta 4021 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 4081 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 4141 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt 4201 aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagatt 4261 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 4321 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 4381 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 4441 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 4501 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 4561 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 4621 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 4681 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 4741 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 4801 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 4861 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 4921 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 4981 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 5041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 5101 tggctcacct tcgggtgggc ctttctgcgt ttata //