LOCUS BBa_I722011 2702 bp DNA linear 22-Nov-2024 DEFINITION rhl producer followed by a AHL acceptor ACCESSION BBa_I722011 VERSION BBa_I722011.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..683 /label=rhlL misc_feature 684..716 /label=LVA misc_feature 723..747 /label=Help:Barcodes CDS 81..722 /label=BBa_C0070 misc_feature 760..763 /label=conserved RBS 756..767 /label=BBa_B0034 CDS 774..1496 /label=BBa_E0020 stem_loop 1516..1559 /label=stem_loop misc_feature 1505..1584 /label=BBa_B0010 terminator 1505..1584 /label=BBa_B0010 stem_loop 1600..1619 /label=T7 TE misc_feature 1626..1626 /label=stop polyA_site 1620..1633 /label=polya misc_feature 1593..1633 /label=BBa_B0012 terminator 1593..1633 /label=BBa_B0012 misc_feature 1..1633 /label=BBa_I0405 misc_feature 1642..1660 /label=TetR 1 misc_feature 1642..1695 /label=BBa_R0040 promoter 1661..1666 /label=-35 misc_feature 1667..1685 /label=TetR 2 promoter 1684..1689 /label=-10 promoter 1642..1695 /label=BBa_R0040 misc_feature 1708..1711 /label=conserved RBS 1704..1715 /label=BBa_B0034 misc_feature 1722..1723 /label=prefix misc_feature 1895..1895 /label=T misc_feature 2213..2213 /label=A CDS 1722..2471 /label=luxR misc_feature 1722..2477 /label=BBa_C0062 misc_feature 2478..2502 /label=Help:Barcodes CDS 1722..2477 /label=BBa_C0062 stem_loop 2522..2565 /label=stem_loop misc_feature 2511..2590 /label=BBa_B0010 terminator 2511..2590 /label=BBa_B0010 stem_loop 2606..2625 /label=T7 TE misc_feature 2632..2632 /label=stop polyA_site 2626..2639 /label=polya misc_feature 2599..2639 /label=BBa_B0012 terminator 2599..2639 /label=BBa_B0012 terminator 2511..2639 /label=BBa_B0015 misc_feature 2648..2667 /label=LuxR/HSL promoter 2667..2672 /label=-35 promoter 2689..2694 /label=-10 misc_feature 2700..2700 /label=start misc_feature 2648..2702 /label=BBa_R0062 promoter 2648..2702 /label=BBa_R0062 misc_feature 1642..2702 /label=BBa_F2620 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgatcgaac tgctgtccga atccctggaa ggtctgtccg 121 ctgctatgat cgctgaactg ggtcgttacc gtcaccaggt tttcatcgaa aaactgggtt 181 gggacgttgt ttccacctcc cgtgttcgtg accaggagtt cgaccagttc gaccacccgc 241 agacccgtta catcgttgct atgtcccgtc agggtatctg cggttgcgct cgtctgctgc 301 cgaccaccga cgcttacctg ctgaaagacg ttttcgctta cctgtgctcc gaaaccccgc 361 cgtccgaccc gtccgtttgg gaactgtccc gttacgctgc ttccgctgct gacgacccgc 421 agctggctat gaaaatcttc tggtcctccc tccagtgcgc ttggtacctg ggtgcttcct 481 ccgttgttgc tgttaccacc accgctatgg aacgttactt cgttcgtaac ggtgttatcc 541 tccagcgtct gggtccgccg cagaaagtta aaggtgaaac cctggttgct atctccttcc 601 cggcttacca ggaacgtggt ctggaaatgc tgctgcgtta ccacccggaa tggctccagg 661 gtgttccgct gtccatggct gttgctgcaa acgacgaaaa ctacgcttta gtagcttaat 721 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggtga 781 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 841 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 901 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 961 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 1021 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 1081 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 1141 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 1201 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca 1261 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 1321 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 1381 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 1441 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact 1501 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1561 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1621 ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat 1681 agagatactg agcactacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg 1741 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc 1801 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt 1861 atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga 1921 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact 1981 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc 2041 caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc 2101 atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata 2161 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg 2221 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag 2281 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat 2341 taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata 2401 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat 2461 actttaaaaa ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca 2521 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 2581 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 2641 actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata 2701 aa //