LOCUS       BBa_I722015             1877 bp    DNA     linear       09-May-2024
DEFINITION  mRFP and rhIR protein generator
ACCESSION   BBa_I722015
VERSION     BBa_I722015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     misc_feature    1..923
                     /label=BBa_I13521
     misc_feature    932..950
                     /label=TetR 1
     misc_feature    932..985
                     /label=BBa_R0040
     promoter        951..956
                     /label=-35
     misc_feature    957..975
                     /label=TetR 2
     promoter        974..979
                     /label=-10
     promoter        932..985
                     /label=BBa_R0040
     misc_feature    1..985
                     /label=BBa_S03836
     misc_feature    998..1001
                     /label=conserved
     RBS             994..1005
                     /label=BBa_B0034
     CDS             1012..1740
                     /label=BBa_C0171
     stem_loop       1760..1803
                     /label=stem_loop
     misc_feature    1749..1828
                     /label=BBa_B0010
     terminator      1749..1828
                     /label=BBa_B0010
     stem_loop       1844..1863
                     /label=T7 TE
     misc_feature    1870..1870
                     /label=stop
     polyA_site      1864..1877
                     /label=polya
     misc_feature    1837..1877
                     /label=BBa_B0012
     terminator      1837..1877
                     /label=BBa_B0012
     misc_feature    994..1877
                     /label=BBa_I1466
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc
      961 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgaggaat
     1021 gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc gatccacgac
     1081 agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt cgattactac
     1141 gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt ccatggcacc
     1201 tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt ggatccggcg
     1261 atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct gttcgaccag
     1321 agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc gaccttgccg
     1381 atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca gcagaacatc
     1441 tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga gttgctgacc
     1501 cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg cctgagccat
     1561 cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga aatcgccatc
     1621 atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca gaagaagttc
     1681 gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct catctaataa
     1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1861 gcctttctgc gtttata
//