LOCUS BBa_I723023 3572 bp DNA linear 22-Dec-2024 DEFINITION Luciferase reporter activated by xylene ACCESSION BBa_I723023 VERSION BBa_I723023.1 FEATURES Location/Qualifiers stem_loop 2165..2208 /label=stem_loop misc_feature 2154..2233 /label=BBa_B0010 terminator 2154..2233 /label=BBa_B0010 stem_loop 2249..2268 /label=T7 TE misc_feature 2275..2275 /label=stop polyA_site 2269..2282 /label=polya misc_feature 2242..2282 /label=BBa_B0012 terminator 2242..2282 /label=BBa_B0012 RBS 2619..2630 /label=BBa_J61101 misc_feature 2637..3572 /label=Rluc CDS 2637..3572 /label=Rluc misc_feature 2637..3572 /label=BBa_J52008 ORIGIN 1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct 61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca 121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag 181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg 241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca 301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt 361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagta 421 aaaacaagag gaaaacaata ctagatgtcg cttacataca aacccaagat gcagcatgag 481 gatatgcaag accttagcag ccagatccgt ttcgttgccg ccgaaggcaa gatctggttg 541 ggagagcagc gcatgctcgt aatgcagcta tctacgctgg ccagcttccg tcgcgaaatt 601 atcagcttga tcggcgtcga gcgggccaag ggtttcttcc tgcggttggg ctatcagtcc 661 ggcctgatgg atgccgagct ggcacgcaag ctgcggccgg ccatgcgcga ggaggaggtg 721 ttcctggctg ggcctcaatt gtatgcgctc aaggggatgg tcaaagtacg cttgctgaca 781 atggatatcg ccatccggga cggacgtttc aacgtggagg ccgagtggat tgattccttt 841 gaagtggata tctgccgaac tgagctgggc ctgatgaatg agcccgtctg ctggacggtg 901 ctaggctatg ctagcggcta tggttcggca ttcatgggcc gcagaatcat tttccaggaa 961 actagctgtc gcgggtgcgg tgacgataaa tgccttatcg tcggcaagac cgcagaagag 1021 tggggcgatg tcagcagttt cgaagcctac ttcaaaagcg acccgatcgt agacgagcgc 1081 tacgagctgc agacccaggt tgccaacctg cgcaaccgcc tgaagcagta cgatgggcag 1141 tattacggca ttggccattc gccagcctac aagcgcatct gtgagaccat cgacaaggct 1201 gcacgcggca gggtttcggt cctgctactg ggtgagactg gggtgggcaa ggaggtaatc 1261 gcgcgcagcg tgcatttgcg cagtgagcgc gcagagcaac ccttcgtcgc ggtgaactgt 1321 gcggcaattc cgccggatct gatcgagtcg gaactgtttg gtgtcgataa gggcgcctat 1381 acgggcgcgg tcaatgcacg cgctggacgt tttgaacggg ccaacggcgg caccatcttt 1441 cttgatgagg tgatcgaatt gacgccgagg gcccaggcca ccctgctacg ggtattgcag 1501 gaaggagagc tagagcgggt cggcggcgac cgcacgcgaa aggtcgacgt gaggttaatc 1561 accgcaacaa acgagaacct ggaagaggcg gtcaagatgg ggcgctttcg cgcagacctg 1621 ttctttcggc tgaatgtttt tcccgtgcat atcccgccgt tgcgcgagcg cgtggaagat 1681 atcccgctgc tggtcgagca ttttcttaga aggcaccata aggaatacgg taagaagact 1741 cttggcctgt ctgatcgagc gatggaggcc tgcctccact accaatggcc aggcaatatc 1801 cgcgagctgg agaacgccct tgagcgcggg gtgattctta ccgagagcaa cgaaagcatc 1861 aatgtcgagt cgctgttccc ggggttggcg acggctaccg aaggcgacag gctatcgagc 1921 gagggccggt tggaggagga gtccggtgac agttggttta ggcaaattat cgaccagggc 1981 gtcagcctcg aagatctcga agcgggttta atgcgcacgg ccatggaccg ttgtgggcag 2041 aatatctcac aggcggcgcg gttgctggga ttgacccgcc cggcaatggc ctatcgactt 2101 aagaagcttg accccagctt atctgtgaaa gcaatgggcc gatagtacta gagccaggca 2161 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2221 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2281 tatactagag cccgggaaag cgcgatgaac cttttttatc gctgccttga tcaaatcgac 2341 aggtggttat gcgcgattga tgatttgctc aaatacagcc agcgtgctgt agattttctc 2401 tcataccccc cctttctttt ttacaaagaa aatcaataat ttagatgaaa taaggggatc 2461 ggtataagca atggcatggc ggttgctagc tatacgagac ttaaaataaa aatagtggtg 2521 acccttcaat gttgtatttt ctcaactctg ttcagattgg ttgctttcgc catgtatatc 2581 ctcaaagcgg gccagccgta gccgttacgc tactagagaa agacaggacc tactagatgg 2641 cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg 2701 ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc 2761 acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc 2821 acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg 2881 gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca 2941 ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg 3001 gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc 3061 atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg 3121 atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg 3181 tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct 3241 acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg 3301 agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg 3361 cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct 3421 tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga 3481 agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct 3541 tcgtggagcg cgtgctgaag aacgagcagt aa //