LOCUS BBa_I724001 3062 bp DNA linear 28-Nov-2024 DEFINITION LacI + ECFP under luxpR, with autoinducer inactivation ACCESSION BBa_I724001 VERSION BBa_I724001.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 1171..1209 /label=LVA CDS 82..1209 /label=lacI-LVA misc_feature 82..1209 /label=BBa_C0012 misc_feature 1210..1234 /label=Help:Barcodes CDS 82..1209 /label=BBa_C0012 misc_feature 64..1234 /label=BBa_S0100 misc_feature 1247..1250 /label=conserved RBS 1243..1254 /label=BBa_B0034 CDS 1261..2022 /label=CFP (LVA) misc_feature 1979..2016 /label=SsrA misc_feature 2017..2022 /label=2 misc_feature 1329..1329 /label=A misc_feature 1261..2022 /label=BBa_E0022 misc_feature 1261..2022 /label=BBa_E0022 stem_loop 2042..2085 /label=stem_loop misc_feature 2031..2110 /label=BBa_B0010 terminator 2031..2110 /label=BBa_B0010 stem_loop 2126..2145 /label=T7 TE misc_feature 2152..2152 /label=stop polyA_site 2146..2159 /label=polya misc_feature 2119..2159 /label=BBa_B0012 terminator 2119..2159 /label=BBa_B0012 misc_feature 1243..2159 /label=BBa_E0422 misc_feature 1..2159 /label=BBa_J40001 misc_feature 2168..2187 /label=LuxR/HSL promoter 2187..2192 /label=-35 promoter 2209..2214 /label=-10 misc_feature 2220..2220 /label=start misc_feature 2168..2222 /label=BBa_R0062 promoter 2168..2222 /label=BBa_R0062 misc_feature 2235..2238 /label=conserved RBS 2231..2242 /label=BBa_B0034 misc_feature 2249..2251 /label=start misc_feature 3032..3037 /label=2 misc_feature 2999..3031 /label=LVA CDS 2249..2998 /label=aiiA misc_feature 2249..3037 /label=BBa_C0060 misc_feature 3038..3062 /label=Help:Barcodes CDS 2249..3037 /label=BBa_C0060 misc_feature 2168..3062 /label=BBa_I724000 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca 121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta 1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag 1261 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1321 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 1381 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 1441 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag 1501 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 1561 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 1621 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 1681 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac 1741 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 1801 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 1861 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 1921 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg 1981 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca 2041 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 2101 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 2161 actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata 2221 aatactagag aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc 2281 aggtcgttgt atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt 2341 agacttaccg gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac 2401 aggtatgcca gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg 2461 gcaggtttta ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg 2521 ttatgagccg gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg 2581 aggaaatggc gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc 2641 gcagcatagc gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat 2701 tgaaggtgat tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc 2761 agggcatcaa tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga 2821 tgcatcgtat acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga 2881 attagcttta tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt 2941 tttctttgga catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc 3001 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc 3061 gc //