LOCUS       BBa_I724003             2476 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive LuxR Generator + LacI regulated RFP(+LVA) and 
            LuxI(LVA)
ACCESSION   BBa_I724003
VERSION     BBa_I724003.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     CDS             731..1405
                     /label=mrfp1
     misc_feature    1445..1450
                     /label=Stop
     misc_feature    1406..1444
                     /label=LVA
     misc_feature    731..733
                     /label=Start
     misc_feature    731..1450
                     /label=BBa_J04051
     stem_loop       1470..1513
                     /label=stem_loop
     misc_feature    1459..1538
                     /label=BBa_B0010
     terminator      1459..1538
                     /label=BBa_B0010
     promoter        1547..1581
                     /label=BBa_J23119
     misc_feature    1594..1597
                     /label=conserved
     RBS             1590..1601
                     /label=BBa_B0034
     misc_feature    1608..1609
                     /label=prefix
     misc_feature    1781..1781
                     /label=T
     misc_feature    2099..2099
                     /label=A
     CDS             1608..2357
                     /label=luxR
     misc_feature    1608..2363
                     /label=BBa_C0062
     misc_feature    2364..2388
                     /label=Help:Barcodes
     CDS             1608..2363
                     /label=BBa_C0062
     stem_loop       2408..2451
                     /label=stem_loop
     misc_feature    2397..2476
                     /label=BBa_B0010
     terminator      2397..2476
                     /label=BBa_B0010
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      781 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      841 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      901 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      961 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1021 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1081 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1141 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1201 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1261 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1321 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1381 tgaaggtcgt cactccaccg gtgctaggcc tgctgcaaac gacgaaaact acgctttagt
     1441 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagttga cagctagctc
     1561 agtcctaggt ataatgctag ctactagaga aagaggagaa atactagatg aaaaacataa
     1621 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata
     1681 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga
     1741 tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa
     1801 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt
     1861 ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa
     1921 aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc
     1981 ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca
     2041 actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc
     2101 tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca
     2161 aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa
     2221 aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac
     2281 tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt
     2341 gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag
     2401 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2461 gtcggtgaac gctctc
//