LOCUS BBa_I724004 2884 bp DNA linear 22-Nov-2024 DEFINITION Elowitz Repressilator with Degradation tag on Reporter ACCESSION BBa_I724004 VERSION BBa_I724004.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 promoter 1038..1043 /label=-10 promoter 1015..1020 /label=-35 misc_feature 1025..1041 /label=OR1 misc_feature 1001..1017 /label=OR2 misc_feature 1001..1049 /label=BBa_R0051 promoter 1001..1049 /label=BBa_R0051 misc_feature 1062..1065 /label=conserved RBS 1058..1069 /label=BBa_B0034 CDS 1079..1695 /label=tetR misc_feature 1696..1729 /label=SsrA misc_feature 1736..1760 /label=Help:Barcodes CDS 1076..1735 /label=BBa_C0040 stem_loop 1780..1823 /label=stem_loop misc_feature 1769..1848 /label=BBa_B0010 terminator 1769..1848 /label=BBa_B0010 stem_loop 1864..1883 /label=T7 TE misc_feature 1890..1890 /label=stop polyA_site 1884..1897 /label=polya misc_feature 1857..1897 /label=BBa_B0012 terminator 1857..1897 /label=BBa_B0012 misc_feature 1906..1924 /label=TetR 1 misc_feature 1906..1959 /label=BBa_R0040 promoter 1925..1930 /label=-35 misc_feature 1931..1949 /label=TetR 2 promoter 1948..1953 /label=-10 promoter 1906..1959 /label=BBa_R0040 misc_feature 1972..1975 /label=conserved RBS 1968..1979 /label=BBa_B0034 misc_feature 2742..2747 /label=stop misc_feature 2703..2741 /label=AAV misc_feature 1986..2747 /label=BBa_E0034 stem_loop 2767..2810 /label=stem_loop misc_feature 2756..2835 /label=BBa_B0010 terminator 2756..2835 /label=BBa_B0010 stem_loop 2851..2870 /label=T7 TE misc_feature 2877..2877 /label=stop polyA_site 2871..2884 /label=polya misc_feature 2844..2884 /label=BBa_B0012 terminator 2844..2884 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgtc 1081 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1141 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1201 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1261 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1321 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1381 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1441 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1501 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1561 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1621 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1681 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1741 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1801 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 1861 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata 1921 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat 1981 actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 2041 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2101 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2161 ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca 2221 tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2281 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2341 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2401 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 2461 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 2521 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 2581 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 2641 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 2701 agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac tagagccagg 2761 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2821 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2881 tata //