LOCUS BBa_I724005 4263 bp DNA linear 24-Dec-2024 DEFINITION Elowitz repressilator with added degradation tag ACCESSION BBa_I724005 VERSION BBa_I724005.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 misc_feature 67..70 /label=conserved misc_feature 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 81..830 /label=BBa_C0051 misc_feature 831..855 /label=barcode misc_feature 864..943 /label=BBa_B0010 stem_loop 875..918 /label=stem_loop misc_feature 952..992 /label=BBa_B0012 stem_loop 959..978 /label=T7 TE polyA_site 979..992 /label=polya misc_feature 985..985 /label=stop misc_feature 1001..1017 /label=OR2 misc_feature 1001..1049 /label=BBa_R0051 promoter 1015..1020 /label=-35 misc_feature 1025..1041 /label=OR1 promoter 1038..1043 /label=-10 misc_feature 1062..1065 /label=conserved misc_feature 1058..1069 /label=BBa_B0034 CDS 1076..2203 /label=lacI-LVA misc_feature 1076..2203 /label=BBa_C0012 misc_feature 2165..2203 /label=LVA misc_feature 2204..2228 /label=barcode misc_feature 2237..2316 /label=BBa_B0010 stem_loop 2248..2291 /label=stem_loop misc_feature 2325..2365 /label=BBa_B0012 stem_loop 2332..2351 /label=T7 TE polyA_site 2352..2365 /label=polya misc_feature 2358..2358 /label=stop misc_feature 2374..2427 /label=BBa_R0011 misc_feature 2376..2392 /label=lac O1 promoter 2393..2398 /label=-35 misc_feature 2399..2415 /label=lac O1 promoter 2416..2421 /label=-10 misc_feature 2441..2444 /label=conserved misc_feature 2437..2448 /label=BBa_B0034 CDS 2458..3074 /label=tetR misc_feature 3075..3108 /label=SsrA misc_feature 2455..3114 /label=BBa_C0040 misc_feature 3115..3139 /label=barcode misc_feature 3148..3227 /label=BBa_B0010 stem_loop 3159..3202 /label=stem_loop misc_feature 3236..3276 /label=BBa_B0012 stem_loop 3243..3262 /label=T7 TE polyA_site 3263..3276 /label=polya misc_feature 3269..3269 /label=stop misc_feature 3285..3303 /label=TetR 1 misc_feature 3285..3338 /label=BBa_R0040 promoter 3304..3309 /label=-35 misc_feature 3310..3328 /label=TetR 2 promoter 3327..3332 /label=-10 misc_feature 3351..3354 /label=conserved misc_feature 3347..3358 /label=BBa_B0034 misc_feature 4082..4120 /label=AAV misc_feature 4121..4126 /label=stop misc_feature 3365..4126 /label=BBa_E0034 misc_feature 4135..4214 /label=BBa_B0010 stem_loop 4146..4189 /label=stem_loop misc_feature 4223..4263 /label=BBa_B0012 stem_loop 4230..4249 /label=T7 TE polyA_site 4250..4263 /label=polya misc_feature 4256..4256 /label=stop ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt 1081 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 1141 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 1201 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 1261 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 1321 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 1381 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 1441 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 1501 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 1561 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 1621 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 1681 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 1741 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 1801 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 1861 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag 1921 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 1981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 2041 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 2101 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 2161 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2341 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt 2401 gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata ctagatgtcc 2461 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 2521 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 2581 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 2641 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 2701 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 2761 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 2821 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 2881 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 2941 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 3001 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 3061 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 3121 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3181 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3241 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 3301 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 3361 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 3421 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 3481 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 3541 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 3601 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 3661 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 3721 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 3781 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 3841 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 3901 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 3961 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 4021 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 4081 gaggcctgct gcaaacgacg aaaactacgc tgctgctgtt taataatact agagccaggc 4141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 4201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 4261 ata // LOCUS BBa_I724006 4263 bp DNA linear 24-Dec-2024 DEFINITION Standard Elowitz Repressilator with Degradation Tag Addition ACCESSION BBa_I724006 VERSION BBa_I724006.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..855 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 promoter 1038..1043 /label=-10 promoter 1015..1020 /label=-35 misc_feature 1025..1041 /label=OR1 misc_feature 1001..1017 /label=OR2 misc_feature 1001..1049 /label=BBa_R0051 promoter 1001..1049 /label=BBa_R0051 misc_feature 1062..1065 /label=conserved RBS 1058..1069 /label=BBa_B0034 misc_feature 2165..2203 /label=LVA CDS 1076..2203 /label=lacI-LVA misc_feature 1076..2203 /label=BBa_C0012 misc_feature 2204..2228 /label=Help:Barcodes CDS 1076..2203 /label=BBa_C0012 stem_loop 2248..2291 /label=stem_loop misc_feature 2237..2316 /label=BBa_B0010 terminator 2237..2316 /label=BBa_B0010 stem_loop 2332..2351 /label=T7 TE misc_feature 2358..2358 /label=stop polyA_site 2352..2365 /label=polya misc_feature 2325..2365 /label=BBa_B0012 terminator 2325..2365 /label=BBa_B0012 misc_feature 2376..2392 /label=lac O1 promoter 2393..2398 /label=-35 misc_feature 2399..2415 /label=lac O1 promoter 2416..2421 /label=-10 misc_feature 2374..2427 /label=BBa_R0011 promoter 2374..2427 /label=BBa_R0011 misc_feature 2441..2444 /label=conserved RBS 2437..2448 /label=BBa_B0034 CDS 2458..3074 /label=tetR misc_feature 3075..3108 /label=SsrA misc_feature 3115..3139 /label=Help:Barcodes CDS 2455..3139 /label=BBa_C0040 stem_loop 3159..3202 /label=stem_loop misc_feature 3148..3227 /label=BBa_B0010 terminator 3148..3227 /label=BBa_B0010 stem_loop 3243..3262 /label=T7 TE misc_feature 3269..3269 /label=stop polyA_site 3263..3276 /label=polya misc_feature 3236..3276 /label=BBa_B0012 terminator 3236..3276 /label=BBa_B0012 misc_feature 3285..3303 /label=TetR 1 misc_feature 3285..3338 /label=BBa_R0040 promoter 3304..3309 /label=-35 misc_feature 3310..3328 /label=TetR 2 promoter 3327..3332 /label=-10 promoter 3285..3338 /label=BBa_R0040 misc_feature 3351..3354 /label=conserved RBS 3347..3358 /label=BBa_B0034 misc_feature 4121..4126 /label=stop misc_feature 4082..4120 /label=AAV misc_feature 3365..4126 /label=BBa_E0034 stem_loop 4146..4189 /label=stem_loop misc_feature 4135..4214 /label=BBa_B0010 terminator 4135..4214 /label=BBa_B0010 stem_loop 4230..4249 /label=T7 TE misc_feature 4256..4256 /label=stop polyA_site 4250..4263 /label=polya misc_feature 4223..4263 /label=BBa_B0012 terminator 4223..4263 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt 1081 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 1141 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 1201 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 1261 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 1321 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 1381 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 1441 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 1501 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 1561 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 1621 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 1681 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 1741 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 1801 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 1861 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag 1921 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 1981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 2041 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 2101 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 2161 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2341 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt 2401 gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata ctagatgtcc 2461 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 2521 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 2581 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 2641 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 2701 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 2761 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 2821 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 2881 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 2941 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 3001 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 3061 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 3121 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3181 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3241 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 3301 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 3361 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 3421 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 3481 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 3541 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 3601 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 3661 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 3721 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 3781 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 3841 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 3901 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 3961 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 4021 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 4081 gaggcctgct gcaaacgacg aaaactacgc tgctgctgtt taataatact agagccaggc 4141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 4201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 4261 ata //