LOCUS       BBa_I724007             2574 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive LuxR Generator + LacI regulated RFP(+LVA) and 
            LuxI(+LVA)
ACCESSION   BBa_I724007
VERSION     BBa_I724007.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     CDS             731..1405
                     /label=mrfp1
     misc_feature    1445..1450
                     /label=Stop
     misc_feature    1406..1444
                     /label=LVA
     misc_feature    731..733
                     /label=Start
     misc_feature    731..1450
                     /label=BBa_J04051
     stem_loop       1470..1513
                     /label=stem_loop
     misc_feature    1459..1538
                     /label=BBa_B0010
     terminator      1459..1538
                     /label=BBa_B0010
     stem_loop       1554..1573
                     /label=T7 TE
     misc_feature    1580..1580
                     /label=stop
     polyA_site      1574..1587
                     /label=polya
     misc_feature    1547..1587
                     /label=BBa_B0012
     terminator      1547..1587
                     /label=BBa_B0012
     promoter        1596..1630
                     /label=BBa_J23119
     misc_feature    1643..1646
                     /label=conserved
     RBS             1639..1650
                     /label=BBa_B0034
     misc_feature    1657..1658
                     /label=prefix
     misc_feature    1830..1830
                     /label=T
     misc_feature    2148..2148
                     /label=A
     CDS             1657..2406
                     /label=luxR
     misc_feature    1657..2412
                     /label=BBa_C0062
     misc_feature    2413..2437
                     /label=Help:Barcodes
     CDS             1657..2412
                     /label=BBa_C0062
     stem_loop       2457..2500
                     /label=stem_loop
     misc_feature    2446..2525
                     /label=BBa_B0010
     terminator      2446..2525
                     /label=BBa_B0010
     stem_loop       2541..2560
                     /label=T7 TE
     misc_feature    2567..2567
                     /label=stop
     polyA_site      2561..2574
                     /label=polya
     misc_feature    2534..2574
                     /label=BBa_B0012
     terminator      2534..2574
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      781 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      841 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      901 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      961 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1021 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1081 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1141 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1201 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1261 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1321 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1381 tgaaggtcgt cactccaccg gtgctaggcc tgctgcaaac gacgaaaact acgctttagt
     1441 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1561 cttcgggtgg gcctttctgc gtttatatac tagagttgac agctagctca gtcctaggta
     1621 taatgctagc tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac
     1681 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc
     1741 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct
     1801 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa
     1861 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat
     1921 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat
     1981 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg
     2041 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat
     2101 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat
     2161 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa
     2221 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt
     2281 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca
     2341 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt
     2401 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
     2461 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2521 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//