BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation2000 1 -35 range2000 1 20 25 annotation1999 1 lac O1 range1999 1 3 19 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 BBa_I725000 1 BBa_I725000 Ca_20_21 coding region 2007-06-03T11:00:00Z 2015-08-31T04:07:54Z One of three potential genes from the Clostridium acetobutylicum genome putatively encoding this enzymatic function. Two of these are located on the plasmid, and one of these plasmid-localized genes was used to design this sequence. Enoyl-coA hydratase. Also called butyraldehyde dehydrogenase and butanol dehydrogenase, and thus is presumed to catalyze reactions (20) and (21) in the pathway diagram distributed previously. false true _106_ 0 1421 9 Not in stock false Optimized for E. coli expression using AccelerysGene. false Mike Deyholos BBa_I725001 1 BBa_I725001 test 2007-06-03T11:00:00Z 2015-08-31T04:07:54Z test test true false _106_ 0 1421 9 Discontinued false test false Mike Deyholos component1934212 1 BBa_I725000 component1934213 1 BBa_R0011 annotation1934213 1 BBa_R0011 range1934213 1 795 848 annotation1934212 1 BBa_I725000 range1934212 1 1 786 BBa_I725001_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgttaatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_I725000_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgttaa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z