BBa_I725016 1 BBa_I725016 Thiolase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2873 Thiolase from C. acetobutylicum. This enzyme catalyzes the conversion of acetyl coa to acetoacetyl coa in a preliminary step prior to butanol biosythesis. The sequence has been chosen in order to provide the intial reactants for the butanol synthesis pathway at highter concentrations. false false _106_ 0 1824 9 Not in stock false This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA. false Erin Dul BBa_I725016_sequence 1 atgaaagaagttgttatcgcttccgctgttcgtaccgctatcggttcctacggtaaatccctgaaagacgttccggctgttgacctgggtgctaccgctatcaaagaagctgttaaaaaagctggtatcaaaccggaagacgttaacgaagttatcctgggtaacgttctgcaagctggtctgggtcagaacccggctcgtcaggcttccttcaaagctggtctgccggttgaaatcccggctatgaccatcaacaaagtttgcggttccggtctgcgtaccgtttccctggctgctcagatcatcaaagctggtgacgctgacgttatcatcgctggtggtatggaaaacatgtcccgtgctccgtacctggctaacaacgctcgttggggttaccgtatgggtaacgctaaattcgttgacgaaatgatcaccgacggtctgtgggacgctttcaacgactaccacatgggtatcaccgctgaaaacatcgctgaacgttggaacatctcccgtgaagaacaggacgagttcgctctggcttcccagaaaaaagctgaagaagctatcaaatccggtcagttcaaagacgaaatcgttccggttgttatcaaaggtcgtaaaggtgaaaccgttgttgacaccgacgaacacccgcgtttcggttccaccatcgaaggtctggctaaactgaaaccggctttcaaaaaagacggtaccgttaccgctggtaacgcttccggtctgaacgactgcgctgctgttctggttatcatgtccgctgaaaaagctaaagaactgggtgttaaaccgctggctaaaatcgtttcctacggttccgctggtgttgacccggctatcatgggttacggtccgttctacgctaccaaagctgctatcgaaaaagctggttggaccgttgacgaactggacctgatcgaatccaacgaagctttcgctgctcagtccctggctgttgctaaagacctgaaattcgacatgaacaaagttaacgttaacggtggtgctatcgctctgggtcacccgatcggtgcttccggtgctcgtatcctggttaccctggttcacgctatgcagaaacgtgacgctaaaaaaggtctggctaccctgtgcatcggtggtggtcagggtaccgctatcctgctggaaaaatgccatcaccatcaccatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z