BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I725011
1
BBa_I725011
B-hydroxy butyryl coA dehydrogenase
2007-06-17T11:00:00Z
2015-08-31T04:07:54Z
Genomic sequence of Chlostridium acetobutylicum. CAC_2708.
B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag.
false
false
_106_
0
1824
9
It's complicated
false
The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA.
true
Erin Dul
BBa_I725021
1
BBa_I725021
RBS + B-hydroxy butyryl coA dehydrogenase
2007-10-22T11:00:00Z
2015-08-31T04:07:54Z
The cds was E. coli codon optimized from the C. acetobutylicum sequence
This is used to construct an operon for the major metabolic enzymes in the Clostridium acetobutylicum butanoate pathway.
false
false
_106_
0
1839
9
It's complicated
false
Because the RBS is so small, B-hydroxy butyryl coA dehydrogenase was cloned into the plasmid containing B0034.
false
Wayne Materi
component1953390
1
BBa_B0034
component1953391
1
BBa_I725011
annotation1953391
1
BBa_I725011
range1953391
1
19
888
annotation1953390
1
BBa_B0034
range1953390
1
1
12
BBa_I725021_sequence
1
aaagaggagaaatactagatgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_I725011_sequence
1
atgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z