BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I725025 1 BBa_I725025 RBS + Butanol dehydrogenase 2007-10-22T11:00:00Z 2015-08-31T04:07:54Z RBS from B0034, Butanol dehydrogenase is E. coli codon optimized from C. acetobutylicum This is used to construct an operon for the major metabolic enzymes in the Clostridium acetobutylicum butanoate pathway. false false _106_ 0 1839 9 It's complicated false Because B0034 is too small to cutout from a gel, this part was cloned into the plasmid containing B0034. false Wayne Materi component1953410 1 BBa_I725015 component1953409 1 BBa_B0034 annotation1953410 1 BBa_I725015 range1953410 1 19 1206 annotation1953409 1 BBa_B0034 range1953409 1 1 12 BBa_I725015 1 BBa_I725015 Butanol dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome CAC_3298 Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered. false false _106_ 0 1824 9 It's complicated false This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added. false Erin Dul BBa_I725015_sequence 1 atggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac BBa_I725025_sequence 1 aaagaggagaaatactagatggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z