BBa_I725013 1 BBa_I725013 Butyryl CoA dehyrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2712 Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725016 1 BBa_I725016 Thiolase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2873 Thiolase from C. acetobutylicum. This enzyme catalyzes the conversion of acetyl coa to acetoacetyl coa in a preliminary step prior to butanol biosythesis. The sequence has been chosen in order to provide the intial reactants for the butanol synthesis pathway at highter concentrations. false false _106_ 0 1824 9 Not in stock false This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA. false Erin Dul BBa_I725299 1 BBa_I725299 Test part (including pathway and thiolase) 2009-03-01T12:00:00Z 2015-08-31T04:07:54Z Composite built of other pieces Long description test false false _106_ 0 1822 9 Not in stock false Trivial design considerations to keep web software happy. false Douglas Ridgway component2001715 1 BBa_I72512 component2001712 1 BBa_I725011 component2001718 1 BBa_I725013 component2001720 1 BBa_B0034 component2001714 1 BBa_B0034 component2001723 1 BBa_B0034 component2001725 1 BBa_I725016 component2001711 1 BBa_B0034 component2001717 1 BBa_B0034 component2001721 1 BBa_I725014 component2001724 1 BBa_I725015 annotation2001721 1 BBa_I725014 range2001721 1 2923 5520 annotation2001723 1 BBa_B0034 range2001723 1 5529 5540 annotation2001712 1 BBa_I725011 range2001712 1 19 888 annotation2001725 1 BBa_I725016 range2001725 1 6741 7940 annotation2001718 1 BBa_I725013 range2001718 1 1742 2896 annotation2001720 1 BBa_B0034 range2001720 1 2905 2916 annotation2001711 1 BBa_B0034 range2001711 1 1 12 annotation2001714 1 BBa_B0034 range2001714 1 897 908 annotation2001715 1 BBa_I72512 range2001715 1 915 1715 annotation2001717 1 BBa_B0034 range2001717 1 1724 1735 annotation2001724 1 BBa_I725015 range2001724 1 5547 6734 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I72512 1 BBa_I72512 Enoyl-coa hydratase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome. CAC_2712 Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus. true Erin Dul BBa_I725011 1 BBa_I725011 B-hydroxy butyryl coA dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z Genomic sequence of Chlostridium acetobutylicum. CAC_2708. B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA. true Erin Dul BBa_I725014 1 BBa_I725014 Butyraldehyde dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z pSOL plamid of C. acetobutylicum. CAP_0035 This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725015 1 BBa_I725015 Butanol dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome CAC_3298 Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered. false false _106_ 0 1824 9 It's complicated false This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added. false Erin Dul BBa_I725016_sequence 1 atgaaagaagttgttatcgcttccgctgttcgtaccgctatcggttcctacggtaaatccctgaaagacgttccggctgttgacctgggtgctaccgctatcaaagaagctgttaaaaaagctggtatcaaaccggaagacgttaacgaagttatcctgggtaacgttctgcaagctggtctgggtcagaacccggctcgtcaggcttccttcaaagctggtctgccggttgaaatcccggctatgaccatcaacaaagtttgcggttccggtctgcgtaccgtttccctggctgctcagatcatcaaagctggtgacgctgacgttatcatcgctggtggtatggaaaacatgtcccgtgctccgtacctggctaacaacgctcgttggggttaccgtatgggtaacgctaaattcgttgacgaaatgatcaccgacggtctgtgggacgctttcaacgactaccacatgggtatcaccgctgaaaacatcgctgaacgttggaacatctcccgtgaagaacaggacgagttcgctctggcttcccagaaaaaagctgaagaagctatcaaatccggtcagttcaaagacgaaatcgttccggttgttatcaaaggtcgtaaaggtgaaaccgttgttgacaccgacgaacacccgcgtttcggttccaccatcgaaggtctggctaaactgaaaccggctttcaaaaaagacggtaccgttaccgctggtaacgcttccggtctgaacgactgcgctgctgttctggttatcatgtccgctgaaaaagctaaagaactgggtgttaaaccgctggctaaaatcgtttcctacggttccgctggtgttgacccggctatcatgggttacggtccgttctacgctaccaaagctgctatcgaaaaagctggttggaccgttgacgaactggacctgatcgaatccaacgaagctttcgctgctcagtccctggctgttgctaaagacctgaaattcgacatgaacaaagttaacgttaacggtggtgctatcgctctgggtcacccgatcggtgcttccggtgctcgtatcctggttaccctggttcacgctatgcagaaacgtgacgctaaaaaaggtctggctaccctgtgcatcggtggtggtcagggtaccgctatcctgctggaaaaatgccatcaccatcaccatcactaataa BBa_I725014_sequence 1 atgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa BBa_I725013_sequence 1 atggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac BBa_I725015_sequence 1 atggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_I725011_sequence 1 atgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa BBa_I725299_sequence 1 aaagaggagaaatactagatgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcactactagatgaaagaagttgttatcgcttccgctgttcgtaccgctatcggttcctacggtaaatccctgaaagacgttccggctgttgacctgggtgctaccgctatcaaagaagctgttaaaaaagctggtatcaaaccggaagacgttaacgaagttatcctgggtaacgttctgcaagctggtctgggtcagaacccggctcgtcaggcttccttcaaagctggtctgccggttgaaatcccggctatgaccatcaacaaagtttgcggttccggtctgcgtaccgtttccctggctgctcagatcatcaaagctggtgacgctgacgttatcatcgctggtggtatggaaaacatgtcccgtgctccgtacctggctaacaacgctcgttggggttaccgtatgggtaacgctaaattcgttgacgaaatgatcaccgacggtctgtgggacgctttcaacgactaccacatgggtatcaccgctgaaaacatcgctgaacgttggaacatctcccgtgaagaacaggacgagttcgctctggcttcccagaaaaaagctgaagaagctatcaaatccggtcagttcaaagacgaaatcgttccggttgttatcaaaggtcgtaaaggtgaaaccgttgttgacaccgacgaacacccgcgtttcggttccaccatcgaaggtctggctaaactgaaaccggctttcaaaaaagacggtaccgttaccgctggtaacgcttccggtctgaacgactgcgctgctgttctggttatcatgtccgctgaaaaagctaaagaactgggtgttaaaccgctggctaaaatcgtttcctacggttccgctggtgttgacccggctatcatgggttacggtccgttctacgctaccaaagctgctatcgaaaaagctggttggaccgttgacgaactggacctgatcgaatccaacgaagctttcgctgctcagtccctggctgttgctaaagacctgaaattcgacatgaacaaagttaacgttaacggtggtgctatcgctctgggtcacccgatcggtgcttccggtgctcgtatcctggttaccctggttcacgctatgcagaaacgtgacgctaaaaaaggtctggctaccctgtgcatcggtggtggtcagggtaccgctatcctgctggaaaaatgccatcaccatcaccatcactaataa BBa_I72512_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z