Test part (including pathway and thiolase)
Trivial design considerations to keep web software happy.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Genomic sequence of Chlostridium acetobutylicum. CAC_2708.
B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag.
The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA.
Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus.
The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus.
Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus.
The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.
pSOL plamid of C. acetobutylicum. CAP_0035
This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity.
Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered.
This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added.
Thiolase from C. acetobutylicum. This enzyme catalyzes the conversion of acetyl coa to acetoacetyl coa in a preliminary step prior to butanol biosythesis. The sequence has been chosen in order to provide the intial reactants for the butanol synthesis pathway at highter concentrations.
This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA.
Conversion of the iGEM parts registry to SBOL2.1