##gff-version 3
##sequence-region BBa_I728004 1 633
BBa_I728004	.	sequence_feature	154	171	.	+	0	ID=annotation1935088;Name=Linker w/sfi cut site
BBa_I728004	.	tag	91	108	.	+	0	ID=annotation1935085;Name=His Tag
BBa_I728004	.	sequence_feature	628	633	.	+	0	ID=annotation1935082;Name=EcoR1
BBa_I728004	.	CDS	469	627	.	+	0	ID=annotation1935091;Name=A1-S53
BBa_I728004	.	sequence_feature	76	90	.	+	0	ID=annotation1935084;Name=Linker w/ sfi1 cut site
BBa_I728004	.	sequence_feature	7	75	.	+	0	ID=annotation1935083;Name=Native Signal Sequence
BBa_I728004	.	sequence_feature	109	114	.	+	0	ID=annotation1935086;Name=Trypsin Cleavage
BBa_I728004	.	CDS	115	153	.	+	0	ID=annotation1935087;Name=Polystyrene Binding Peptide
BBa_I728004	.	sequence_feature	457	468	.	+	0	ID=annotation1935090;Name=N-C Terminus Linker
BBa_I728004	.	CDS	172	456	.	+	0	ID=annotation1935089;Name=S54-F148
BBa_I728004	.	sequence_feature	1	6	.	+	0	ID=annotation1935081;Name=xBa1
>BBa_I728004
tctagaatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggcc
agtctggccagcaccaccaccaccaccacaaacgtttcttctctttcttcttcccggcttctgcttggggttctggtgg
ccagtctggccagtctggtgactacaacaaaaaccagtactacggcatcactgctggtccggcttaccgcattaacgac
tgggcaagcatctacggtgtagtgggtgtgggttatggtaaattccagaccactgaatacccgacctacaaacacgaca
ccagcgactacggtttctcctacggtgcgggtctgcagttcaacccgatggaaaacgttgctctggacttctcttacga
gcagagccgtattcgtagcgttgacgtaggcacctggattgccggtgttggttaccgcttcggtggttctggtgcgact
tctactgtaactggcggttacgcacagagcgacgctcagggccaaatgaacaaaatgggcggtttcaacctgaaatacc
gctatgaagaagacaacagcccgctgggtgtgatcggttctttcacttacaccgagaaaagccgtactgcaagcgaattc
c