##gff-version 3
##sequence-region BBa_I728201 1 820
BBa_I728201	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I728201	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I728201	.	CDS	64	717	.	+	0	ID=BBa_I728500;Name=BBa_I728500
BBa_I728201	.	sequence_feature	148	153	.	+	0	ID=annotation1940509;Name=BglII Cut Site;Parent=BBa_I728500
BBa_I728201	.	sequence_feature	133	147	.	+	0	ID=annotation1940502;Name=SfiI Restriction Site;Parent=BBa_I728500
BBa_I728201	.	start_codon	64	66	.	+	0	ID=annotation1940500;Name=Start codon;Parent=BBa_I728500
BBa_I728201	.	CDS	199	204	.	+	0	ID=annotation1940512;Name=Trypsin Cleavage Site;Parent=BBa_I728500
BBa_I728201	.	tag	205	237	.	+	0	ID=annotation1940510;Name=T7 Antibody Tag;Parent=BBa_I728500
BBa_I728201	.	sequence_feature	238	255	.	+	0	ID=annotation1940504;Name=Linker;Parent=BBa_I728500
BBa_I728201	.	sequence_feature	541	552	.	+	0	ID=annotation1940506;Name=Linker;Parent=BBa_I728500
BBa_I728201	.	sequence_feature	193	198	.	+	0	ID=annotation1940511;Name=SalI Restriction Site;Parent=BBa_I728500
BBa_I728201	.	stop_codon	712	717	.	+	0	ID=annotation1940508;Name=Double stop codon (TGATAA);Parent=BBa_I728500
BBa_I728201	.	CDS	64	132	.	+	0	ID=annotation1940501;Name=Native Starting Signal;Parent=BBa_I728500
BBa_I728201	.	CDS	553	711	.	+	0	ID=annotation1940507;Name=A1-S53 of OmpX;Parent=BBa_I728500
BBa_I728201	.	CDS	256	540	.	+	0	ID=annotation1940505;Name=S54-F148 of OmpX;Parent=BBa_I728500
BBa_I728201	.	CDS	64	717	.	+	0	ID=annotation1975607;Name=Coding sequence;Parent=BBa_I728500
BBa_I728201	.	CDS	154	192	.	+	0	ID=annotation1940503;Name=Polystyrene Sticky Peptide;Parent=BBa_I728500
BBa_I728201	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_I728201	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I728201	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I728201	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I728201	.	terminator	726	820	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_I728201	.	terminator	775	820	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_I728201	.	stem_loop	787	809	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I728201	.	engineered_region	775	820	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I728201	.	terminator	726	766	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_I728201	.	engineered_region	726	766	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I728201	.	polyA_site	753	766	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I728201	.	stop_codon	759	759	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I728201	.	stem_loop	733	752	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I728201
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaaaattgcgt
gcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatctttttt
cagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggt
ggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtatta
acgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaaca
cgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagc
tatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtg
cgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaa
atatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagc
tgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagc
cagattattaatccggcttttttattattt