BBa_I728201
1
BBa_I728201
Complex System
2007-10-19T11:00:00Z
2015-08-31T04:07:55Z
The part is made from standard BioBrick and our CPX sequence
The complex system version of our product. It has a promoter, RBS, our CPX surface display mechanism, and a terminator.
false
true
_138_
0
1695
9
It's complicated
false
See BBa_I728500 for more information.
false
Toan Tran-Phu
component2222065
1
BBa_B0034
component2222063
1
BBa_I14032
component2222087
1
BBa_B0014
component2222080
1
BBa_I728500
annotation2222065
1
BBa_B0034
range2222065
1
46
57
annotation2222080
1
BBa_I728500
range2222080
1
64
717
annotation2222063
1
BBa_I14032
range2222063
1
1
37
annotation2222087
1
BBa_B0014
range2222087
1
726
820
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_I728500
1
CPX
CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide
2007-07-30T11:00:00Z
2015-08-31T04:07:55Z
Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968
This will be filled in later.
false
false
_138_
0
1695
9
It's complicated
false
Max of XXXX amino acid long peptide.
true
Toan Tran-Phu
annotation1940502
1
SfiI Restriction Site
range1940502
1
70
84
annotation1940509
1
BglII Cut Site
range1940509
1
85
90
annotation1940512
1
Trypsin Cleavage Site
range1940512
1
136
141
annotation1940504
1
Linker
range1940504
1
175
192
annotation1940506
1
Linker
range1940506
1
478
489
annotation1940510
1
T7 Antibody Tag
range1940510
1
142
174
annotation1940508
1
Double stop codon (TGATAA)
range1940508
1
649
654
annotation1940501
1
Native Starting Signal
range1940501
1
1
69
annotation1940511
1
SalI Restriction Site
range1940511
1
130
135
annotation1940507
1
A1-S53 of OmpX
range1940507
1
490
648
annotation1940500
1
Start codon
range1940500
1
1
3
annotation1940503
1
Polystyrene Sticky Peptide
range1940503
1
91
129
annotation1975607
1
Coding sequence
range1975607
1
1
654
annotation1940505
1
S54-F148 of OmpX
range1940505
1
193
477
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028344
1
-10
range1028344
1
26
31
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
annotation1028343
1
-35
range1028343
1
3
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I728500_sequence
1
atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataa
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_I728201_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z