LOCUS       BBa_I728500              654 bp    DNA     linear       09-May-2024
DEFINITION  CPX Terminal Surface Display Protein with Polystyrene-Binding 
            Peptide
ACCESSION   BBa_I728500
VERSION     BBa_I728500.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start codon
     CDS             1..69
                     /label=Native Starting Signal
     misc_feature    70..84
                     /label=SfiI Restriction Site
     CDS             91..129
                     /label=Polystyrene Sticky Peptide
     misc_feature    175..192
                     /label=Linker
     CDS             193..477
                     /label=S54-F148 of OmpX
     misc_feature    478..489
                     /label=Linker
     CDS             490..648
                     /label=A1-S53 of OmpX
     misc_feature    649..654
                     /label=Double stop codon (TGATAA)
     misc_feature    85..90
                     /label=BglII Cut Site
     misc_feature    142..174
                     /label=T7 Antibody Tag
     misc_feature    130..135
                     /label=SalI Restriction Site
     CDS             136..141
                     /label=Trypsin Cleavage Site
     CDS             1..654
                     /label=Coding sequence
ORIGIN
        1 atgaaaaaaa ttgcgtgcct gagcgcgctg gccgcggttc tggcctttac cgcgggcacc
       61 agcgttgcgg gccagtctgg ccaaagatct tttttcagct ttttttttcc ggcgagcgcg
      121 tggggtagcg tcgacaaacg tatggcgagc atgaccggcg gtcagcagat gggtggtggc
      181 cagagcggcc agagcggtga ttataacaaa aaccagtatt atggcattac cgcgggtccg
      241 gcgtatcgta ttaacgattg ggcgagcatt tatggcgtgg tgggcgtggg ctatggcaaa
      301 tttcagacca ccgaatatcc gacctataaa cacgatacca gcgattatgg ctttagctat
      361 ggcgcgggtc tgcaatttaa tccgatggaa aacgtggcgc tggattttag ctatgaacag
      421 agccgtattc gtagcgtgga tgtgggcacc tggattgcgg gcgtgggtta tcgttttggc
      481 ggcagcggtg cgaccagcac cgtgaccggc ggttatgcgc agagcgatgc gcagggccag
      541 atgaacaaaa tgggcggctt caacctgaaa tatcgctatg aagaagataa cagcccgctg
      601 ggcgtgattg gcagctttac ctataccgaa aaaagccgta ccgcgagctg ataa
//