LOCUS       BBa_I728654             1484 bp    DNA     linear       27-Jun-2024
DEFINITION  Mercury-Induced RFP Reporter System
ACCESSION   BBa_I728654
VERSION     BBa_I728654.1  
FEATURES             Location/Qualifiers
     CDS             54..479
                     /label=MerR
     promoter        480..560
                     /label=-35 and -10 hexamers
     CDS             561..628
                     /label=MerT (first part)
     promoter        1..635
                     /label=BBa_I728456
     CDS             642..1316
                     /label=mrfp1
     misc_feature    1323..1347
                     /label=Help:Barcodes
     CDS             642..1322
                     /label=BBa_E1010
     stem_loop       1367..1410
                     /label=stem_loop
     misc_feature    1356..1435
                     /label=BBa_B0010
     terminator      1356..1435
                     /label=BBa_B0010
     stem_loop       1451..1470
                     /label=T7 TE
     misc_feature    1477..1477
                     /label=stop
     polyA_site      1471..1484
                     /label=polya
     misc_feature    1444..1484
                     /label=BBa_B0012
     terminator      1444..1484
                     /label=BBa_B0012
     misc_feature    642..1484
                     /label=BBa_J04650
ORIGIN
        1 ctctttgacc aggctttcta aggggtcgtc tcagaaaacg gaaaataaag cacgctaagg
       61 catagccgaa cctgccaagc ttgctccacc ctgtagtgac gcgatcagcg ggcaggaaac
      121 gttccccctt cgcgcatggc aggcgcacac caactcagac agcacggcct ccatgcgcgc
      181 caggtcagcc attttctcgc gcacgtcctt gagcttgtgc tcggccagac tgctggcttc
      241 ctcgcaatgg gtgccatcct ccagccgcag cagctcggcg atctcatcca ggctgaagcc
      301 cagccgctgg gctgatttca cgaagcgcac ccgcgttaca tccgcctcgc catagcggcg
      361 gatgctgcca tagggcttgt caggctccag caacaagccc ttgcgctgat agaaacggat
      421 ggtctccaca ttgaccccgg ccgccttggc gaaaacgcca atggtcaggt tctccaaatt
      481 gttttccata tcgcttgact ccgtacatga gtacggaagt aaggttacgc tatccaattt
      541 caattcgaaa ggacaagcgc atgtctgaac caaaaaccgg gcgcggcgcg ctcttcactg
      601 gagggcttgc cgctatcctt gcttctgctt gttagtacta gatggcttcc tccgaagacg
      661 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      721 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      781 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      841 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
      901 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
      961 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1021 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1081 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1141 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1201 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1261 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1321 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1381 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1441 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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