LOCUS       BBa_I728978             1741 bp    DNA     linear       29-Nov-2024
DEFINITION  Promoter+RBS+CPX(Circularly-Permuted OmpX for Surface Display of 
            Polypeptides)
ACCESSION   BBa_I728978
VERSION     BBa_I728978.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     misc_feature    1088..1090
                     /label=Start codon
     CDS             1088..1156
                     /label=Native Starting Signal
     misc_feature    1157..1171
                     /label=SfiI Restriction Site
     CDS             1178..1216
                     /label=Polystyrene Sticky Peptide
     misc_feature    1262..1279
                     /label=Linker
     CDS             1280..1564
                     /label=S54-F148 of OmpX
     misc_feature    1565..1576
                     /label=Linker
     CDS             1577..1735
                     /label=A1-S53 of OmpX
     misc_feature    1736..1741
                     /label=Double stop codon (TGATAA)
     misc_feature    1172..1177
                     /label=BglII Cut Site
     misc_feature    1229..1261
                     /label=T7 Antibody Tag
     misc_feature    1217..1222
                     /label=SalI Restriction Site
     CDS             1223..1228
                     /label=Trypsin Cleavage Site
     CDS             1088..1741
                     /label=Coding sequence
     CDS             1088..1741
                     /label=BBa_I728500
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg aaaaaaattg cgtgcctgag cgcgctggcc gcggttctgg cctttaccgc
     1141 gggcaccagc gttgcgggcc agtctggcca aagatctttt ttcagctttt tttttccggc
     1201 gagcgcgtgg ggtagcgtcg acaaacgtat ggcgagcatg accggcggtc agcagatggg
     1261 tggtggccag agcggccaga gcggtgatta taacaaaaac cagtattatg gcattaccgc
     1321 gggtccggcg tatcgtatta acgattgggc gagcatttat ggcgtggtgg gcgtgggcta
     1381 tggcaaattt cagaccaccg aatatccgac ctataaacac gataccagcg attatggctt
     1441 tagctatggc gcgggtctgc aatttaatcc gatggaaaac gtggcgctgg attttagcta
     1501 tgaacagagc cgtattcgta gcgtggatgt gggcacctgg attgcgggcg tgggttatcg
     1561 ttttggcggc agcggtgcga ccagcaccgt gaccggcggt tatgcgcaga gcgatgcgca
     1621 gggccagatg aacaaaatgg gcggcttcaa cctgaaatat cgctatgaag aagataacag
     1681 cccgctgggc gtgattggca gctttaccta taccgaaaaa agccgtaccg cgagctgata
     1741 a
//