LOCUS       BBa_I728979              820 bp    DNA     linear       22-Dec-2024
DEFINITION  I14032+B0034+BBa_I728000+B0014
ACCESSION   BBa_I728979
VERSION     BBa_I728979.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..66
                     /label=Start codon
     CDS             64..132
                     /label=Native Starting Signal
     misc_feature    133..147
                     /label=SfiI Restriction Site
     CDS             154..192
                     /label=Polystyrene Sticky Peptide
     misc_feature    238..255
                     /label=Linker
     CDS             256..540
                     /label=S54-F148 of OmpX
     misc_feature    541..552
                     /label=Linker
     CDS             553..711
                     /label=A1-S53 of OmpX
     misc_feature    712..717
                     /label=Double stop codon (TGATAA)
     misc_feature    148..153
                     /label=BglII Cut Site
     misc_feature    205..237
                     /label=T7 Antibody Tag
     misc_feature    193..198
                     /label=SalI Restriction Site
     CDS             199..204
                     /label=Trypsin Cleavage Site
     CDS             64..717
                     /label=Coding sequence
     CDS             64..717
                     /label=BBa_I728500
     stem_loop       733..752
                     /label=T7 TE
     misc_feature    759..759
                     /label=stop
     polyA_site      753..766
                     /label=polya
     misc_feature    726..766
                     /label=BBa_B0012
     terminator      726..766
                     /label=BBa_B0012
     stem_loop       787..809
                     /label=stem_loop
     misc_feature    775..820
                     /label=BBa_B0011
     terminator      775..820
                     /label=BBa_B0011
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa aaattgcgtg cctgagcgcg ctggccgcgg ttctggcctt taccgcgggc
      121 accagcgttg cgggccagtc tggccaaaga tcttttttca gctttttttt tccggcgagc
      181 gcgtggggta gcgtcgacaa acgtatggcg agcatgaccg gcggtcagca gatgggtggt
      241 ggccagagcg gccagagcgg tgattataac aaaaaccagt attatggcat taccgcgggt
      301 ccggcgtatc gtattaacga ttgggcgagc atttatggcg tggtgggcgt gggctatggc
      361 aaatttcaga ccaccgaata tccgacctat aaacacgata ccagcgatta tggctttagc
      421 tatggcgcgg gtctgcaatt taatccgatg gaaaacgtgg cgctggattt tagctatgaa
      481 cagagccgta ttcgtagcgt ggatgtgggc acctggattg cgggcgtggg ttatcgtttt
      541 ggcggcagcg gtgcgaccag caccgtgacc ggcggttatg cgcagagcga tgcgcagggc
      601 cagatgaaca aaatgggcgg cttcaacctg aaatatcgct atgaagaaga taacagcccg
      661 ctgggcgtga ttggcagctt tacctatacc gaaaaaagcc gtaccgcgag ctgataatac
      721 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
      781 tataaaaagc cagattatta atccggcttt tttattattt
//