LOCUS BBa_I728999 2977 bp DNA linear 25-Nov-2024 DEFINITION Test part: oscillator made of three random inverters ACCESSION BBa_I728999 VERSION BBa_I728999.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..768 /label=BBa_C0051 stem_loop 813..856 /label=stem_loop misc_feature 802..881 /label=BBa_B0010 terminator 802..881 /label=BBa_B0010 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 promoter 976..981 /label=-10 promoter 953..958 /label=-35 misc_feature 963..979 /label=OR1 misc_feature 939..955 /label=OR2 misc_feature 939..987 /label=BBa_R0051 promoter 939..987 /label=BBa_R0051 misc_feature 1..987 /label=BBa_Q04510 misc_feature 1000..1003 /label=conserved RBS 996..1007 /label=BBa_B0034 CDS 1017..1724 /label=cI lambda misc_feature 1725..1757 /label=LVA misc_feature 1764..1788 /label=Help:Barcodes CDS 1014..1763 /label=BBa_C0051 stem_loop 1808..1851 /label=stem_loop misc_feature 1797..1876 /label=BBa_B0010 terminator 1797..1876 /label=BBa_B0010 stem_loop 1892..1911 /label=T7 TE misc_feature 1918..1918 /label=stop polyA_site 1912..1925 /label=polya misc_feature 1885..1925 /label=BBa_B0012 terminator 1885..1925 /label=BBa_B0012 promoter 1971..1976 /label=-10 promoter 1948..1953 /label=-35 misc_feature 1958..1974 /label=OR1 misc_feature 1934..1950 /label=OR2 misc_feature 1934..1982 /label=BBa_R0051 promoter 1934..1982 /label=BBa_R0051 misc_feature 996..1982 /label=BBa_Q04510 misc_feature 1995..1998 /label=conserved RBS 1991..2002 /label=BBa_B0034 CDS 2012..2719 /label=cI lambda misc_feature 2720..2752 /label=LVA misc_feature 2759..2783 /label=Help:Barcodes CDS 2009..2758 /label=BBa_C0051 stem_loop 2803..2846 /label=stem_loop misc_feature 2792..2871 /label=BBa_B0010 terminator 2792..2871 /label=BBa_B0010 stem_loop 2887..2906 /label=T7 TE misc_feature 2913..2913 /label=stop polyA_site 2907..2920 /label=polya misc_feature 2880..2920 /label=BBa_B0012 terminator 2880..2920 /label=BBa_B0012 promoter 2966..2971 /label=-10 promoter 2943..2948 /label=-35 misc_feature 2953..2969 /label=OR1 misc_feature 2929..2945 /label=OR2 misc_feature 2929..2977 /label=BBa_R0051 promoter 2929..2977 /label=BBa_R0051 misc_feature 1991..2977 /label=BBa_Q04510 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat 961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgagca 1021 caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt 1081 atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga 1141 tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg 1201 ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca 1261 gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg 1321 agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta 1381 ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct 1441 ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg 1501 acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag 1561 ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt 1621 ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg 1681 tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa 1741 actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag 1801 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1861 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1921 ttatatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt 1981 gctactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga 2041 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg 2101 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt 2161 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa 2221 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc 2281 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca 2341 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt 2401 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat 2461 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc 2521 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac 2581 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta 2641 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg 2701 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg 2761 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag 2821 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2881 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg 2941 tgttgactat tttacctctg gcggtgataa tggttgc //