LOCUS       BBa_I729004             1920 bp    DNA     linear       16-May-2024
DEFINITION  Sensitive AHL Receiver
ACCESSION   BBa_I729004
VERSION     BBa_I729004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..836
                     /label=BBa_I729001
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
     misc_feature    982..1001
                     /label=LuxR/HSL
     promoter        1001..1006
                     /label=-35
     promoter        1023..1028
                     /label=-10
     misc_feature    1034..1034
                     /label=start
     misc_feature    982..1036
                     /label=BBa_R0062
     promoter        982..1036
                     /label=BBa_R0062
     RBS             1045..1057
                     /label=RBS-3\Weak
     RBS             1051..1054
                     /label=RBS
     misc_feature    1045..1057
                     /label=BBa_B0032
     RBS             1045..1057
                     /label=BBa_B0032
     CDS             1064..1783
                     /label=GFP protein
     CDS             1064..1783
                     /label=BBa_E0040
     stem_loop       1803..1846
                     /label=stem_loop
     misc_feature    1792..1871
                     /label=BBa_B0010
     terminator      1792..1871
                     /label=BBa_B0010
     stem_loop       1887..1906
                     /label=T7 TE
     misc_feature    1913..1913
                     /label=stop
     polyA_site      1907..1920
                     /label=polya
     misc_feature    1880..1920
                     /label=BBa_B0012
     terminator      1880..1920
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgttcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataatact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt
     1021 gttatagtcg aataaatact agagtcacac aggaaagtac tagatgcgta aaggagaaga
     1081 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1141 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1201 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     1261 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     1321 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1381 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1441 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1501 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1561 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1621 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1681 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1741 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1861 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//