##gff-version 3
##sequence-region BBa_I729004 1 1920
BBa_I729004	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I729004	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I729004	.	CDS	81	836	.	+	0	ID=BBa_I729001;Name=BBa_I729001
BBa_I729004	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I729004	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I729004	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I729004	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I729004	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I729004	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I729004	.	ribosome_entry_site	1045	1057	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I729004	.	engineered_region	1045	1057	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I729004	.	ribosome_entry_site	1045	1057	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I729004	.	ribosome_entry_site	1051	1054	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I729004	.	terminator	845	924	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I729004	.	stem_loop	856	899	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I729004	.	engineered_region	845	924	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I729004	.	terminator	1792	1871	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I729004	.	stem_loop	1803	1846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I729004	.	engineered_region	1792	1871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I729004	.	terminator	1880	1920	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I729004	.	engineered_region	1880	1920	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I729004	.	stop_codon	1913	1913	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I729004	.	polyA_site	1907	1920	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I729004	.	stem_loop	1887	1906	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I729004	.	promoter	982	1036	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I729004	.	non_covalent_binding_site	982	1001	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I729004	.	promoter	1023	1028	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I729004	.	promoter	1001	1006	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I729004	.	engineered_region	982	1036	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I729004	.	start_codon	1034	1034	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I729004	.	terminator	933	973	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I729004	.	engineered_region	933	973	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I729004	.	stop_codon	966	966	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I729004	.	polyA_site	960	973	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I729004	.	stem_loop	940	959	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I729004	.	CDS	1064	1783	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I729004	.	CDS	1064	1783	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_I729004
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgttcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatag
tcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattc
ttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaa
acttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggt
gttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtac
aggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgt
taatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaag
atggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacca
ttacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaca
gctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata