LOCUS       BBa_I729005             2650 bp    DNA     linear       16-Feb-2025
DEFINITION  AHL Reporter and Quencher
ACCESSION   BBa_I729005
VERSION     BBa_I729005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     misc_feature    1088..1090
                     /label=start
     misc_feature    1871..1876
                     /label=2
     misc_feature    1838..1870
                     /label=LVA
     CDS             1088..1837
                     /label=aiiA
     misc_feature    1088..1876
                     /label=BBa_C0060
     misc_feature    1877..1901
                     /label=Help:Barcodes
     CDS             1088..1876
                     /label=BBa_C0060
     misc_feature    1070..1901
                     /label=BBa_C0260
     RBS             1910..1924
                     /label=RBS-1\Strong
     RBS             1917..1921
                     /label=RBS
     misc_feature    1910..1924
                     /label=BBa_B0030
     RBS             1910..1924
                     /label=BBa_B0030
     CDS             1931..2650
                     /label=GFP protein
     CDS             1931..2650
                     /label=BBa_E0040
     misc_feature    1910..2650
                     /label=BBa_E5500
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg acagtaaaga agctttattt cgtcccagca ggtcgttgta tgttggatca
     1141 ttcgtctgtt aatagtacat taacaccagg agaattatta gacttaccgg tttggtgtta
     1201 tcttttggag actgaagaag gacctatttt agtagataca ggtatgccag aaagtgcagt
     1261 taataatgaa ggtcttttta acggtacatt tgtcgaaggg caggttttac cgaaaatgac
     1321 tgaagaagat agaatcgtga atattttaaa acgggttggt tatgagccgg aagaccttct
     1381 ttatattatt agttctcact tgcattttga tcatgcagga ggaaatggcg cttttataaa
     1441 tacaccaatc attgtacagc gtgctgaata tgaggcggcg cagcatagcg aagaatattt
     1501 gaaagaatgt atattgccga atttaaacta caaaatcatt gaaggtgatt atgaagtcgt
     1561 accaggagtt caattattgc atacaccagg ccatactcca gggcatcaat cgctattaat
     1621 tgagacagaa aaatccggtc ctgtattatt aacgattgat gcatcgtata cgaaagagaa
     1681 ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa ttagctttat cttcaattaa
     1741 acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt ttctttggac atgatataga
     1801 gcaggaaagg ggatgtaaag tgttccctga atatatagct gcaaacgacg aaaactacgc
     1861 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagaga ttaaagagga
     1921 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1981 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2041 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     2101 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     2161 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     2221 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     2281 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     2341 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     2401 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     2461 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     2521 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     2581 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     2641 caaataataa
//