LOCUS BBa_I729006 2985 bp DNA linear 22-Dec-2024 DEFINITION AHL reporter and aiia device ACCESSION BBa_I729006 VERSION BBa_I729006.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 RBS 1070..1082 /label=RBS-3\Weak RBS 1076..1079 /label=RBS misc_feature 1070..1082 /label=BBa_B0032 RBS 1070..1082 /label=BBa_B0032 CDS 1089..1808 /label=GFP protein CDS 1089..1808 /label=BBa_E0040 stem_loop 1828..1871 /label=stem_loop misc_feature 1817..1896 /label=BBa_B0010 terminator 1817..1896 /label=BBa_B0010 stem_loop 1912..1931 /label=T7 TE misc_feature 1938..1938 /label=stop polyA_site 1932..1945 /label=polya misc_feature 1905..1945 /label=BBa_B0012 terminator 1905..1945 /label=BBa_B0012 misc_feature 1..1945 /label=BBa_T9002 misc_feature 1956..1972 /label=lac O1 promoter 1973..1978 /label=-35 misc_feature 1979..1995 /label=lac O1 promoter 1996..2001 /label=-10 misc_feature 1954..2007 /label=BBa_R0011 promoter 1954..2007 /label=BBa_R0011 misc_feature 2021..2024 /label=conserved RBS 2017..2028 /label=BBa_B0034 misc_feature 2035..2037 /label=start misc_feature 2818..2823 /label=2 misc_feature 2785..2817 /label=LVA CDS 2035..2784 /label=aiiA misc_feature 2035..2823 /label=BBa_C0060 misc_feature 2824..2848 /label=Help:Barcodes CDS 2035..2823 /label=BBa_C0060 stem_loop 2868..2911 /label=stem_loop misc_feature 2857..2936 /label=BBa_B0010 terminator 2857..2936 /label=BBa_B0010 stem_loop 2952..2971 /label=T7 TE misc_feature 2978..2978 /label=stop polyA_site 2972..2985 /label=polya misc_feature 2945..2985 /label=BBa_B0012 terminator 2945..2985 /label=BBa_B0012 misc_feature 2017..2985 /label=BBa_I0460 misc_feature 1954..2985 /label=BBa_S01656 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa 1081 agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 1801 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1861 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1921 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt 1981 gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata ctagatgaca 2041 gtaaagaagc tttatttcgt cccagcaggt cgttgtatgt tggatcattc gtctgttaat 2101 agtacattaa caccaggaga attattagac ttaccggttt ggtgttatct tttggagact 2161 gaagaaggac ctattttagt agatacaggt atgccagaaa gtgcagttaa taatgaaggt 2221 ctttttaacg gtacatttgt cgaagggcag gttttaccga aaatgactga agaagataga 2281 atcgtgaata ttttaaaacg ggttggttat gagccggaag accttcttta tattattagt 2341 tctcacttgc attttgatca tgcaggagga aatggcgctt ttataaatac accaatcatt 2401 gtacagcgtg ctgaatatga ggcggcgcag catagcgaag aatatttgaa agaatgtata 2461 ttgccgaatt taaactacaa aatcattgaa ggtgattatg aagtcgtacc aggagttcaa 2521 ttattgcata caccaggcca tactccaggg catcaatcgc tattaattga gacagaaaaa 2581 tccggtcctg tattattaac gattgatgca tcgtatacga aagagaattt tgaaaatgaa 2641 gtgccatttg cgggatttga ttcagaatta gctttatctt caattaaacg tttaaaagaa 2701 gtggtgatga aagagaagcc gattgttttc tttggacatg atatagagca ggaaagggga 2761 tgtaaagtgt tccctgaata tatagctgca aacgacgaaa actacgcttt agtagcttaa 2821 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 2881 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2941 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //